Hye Ryun Woo23, Hee Jung Koo2, Jeongsik Kim2, Hyobin Jeong2, Jin Ok Yang, Il Hwan Lee, Ji Hyung Jun, Seung Hee Choi, Su Jin Park, Byeongsoo Kang, You Wang Kim, Bong-Kwan Phee, Jin Hee Kim, Chaehwa Seo, Charny Park, Sang Cheol Kim, Seongjin Park, Byungwook Lee, Sanghyuk Lee, Daehee Hwang3, Hong Gil Nam3 and Pyung Ok Lim3*
Department of New Biology, DGIST, Daegu, Republic of Korea (H.R.W., D.H., H.G.N., P.O.L.);
Center for Plant Aging Research, Institute for Basic Science (IBS), Daegu, Republic of Korea (H.J.K., J.K., H.J., I.H.L., S.H.C., S.J.P., B.K., Y.W.K., B.-K.P., J.H.K., D.H., H.G.N.);
School of Interdisciplinary Bioscience and Bioengineering, POSTECH, Pohang, Republic of Korea (H.J.K.);
Korean Bioinformation Center, KRIBB, Daejeon, Republic of Korea (J.O.Y., S.C.K., S.P., B.L.);
Division of Molecular Life Sciences, POSTECH, Pohang, Republic of Korea (I.H.L., J.H.J., S.H.C.);
Division of Integrative Biosciences and Biotechnologies, POSTECH, Pohang, Republic of Korea (S.J.P.);
DNA Link Inc., Seoul, Republic of Korea (C.S.); and
Ewha Research Center for Systems Biology and Department of Life Science, Ewha Womans University, Seoul, Republic of Korea (C.P., S.L.)
Plant leaves, harvesting light energy and fixing CO2, are a major source of foods on the earth. Leaves undergo developmental and physiological shifts during their lifespan, ending with senescence and death. We characterized the key regulatory features of the leaf transcriptome during aging by analyzing total- and small-RNA transcriptomes throughout the lifespan of Arabidopsis (Arabidopsis thaliana) leaves at multidimensions, including age, RNA-type, and organelle. Intriguingly, senescing leaves showed more coordinated temporal changes in transcriptomes than growing leaves, with sophisticated regulatory networks comprising transcription factors and diverse small regulatory RNAs. The chloroplast transcriptome, but not the mitochondrial transcriptome, showed major changes during leaf aging, with a strongly shared expression pattern of nuclear transcripts encoding chloroplast-targeted proteins. Thus, unlike animal aging, leaf senescence proceeds with tight temporal and distinct interorganellar coordination of various transcriptomes that would be critical for the highly regulated degeneration and nutrient recycling contributing to plant fitness and productivity.
2 These authors contributed equally to the article.
3 These authors jointly supervised this work.
* Address correspondence to P.O.L.