한빛사 논문
Johns Hopkins University School of Medicine, Fred Hutchinson Cancer Research Center
Abstract
Youn Na,1,2,6 Sungjin Park,1,4,6 Changhee Lee,1,3 Dong-Kyu Kim,4 Joo Min Park,1,5 Shanthini Sockanathan,1 Richard L. Huganir,1 and Paul F. Worley1,*
1Solomon Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
2Divison of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
3Department of Genetics, Harvard Medical School, Boston, MA 02446, USA
4Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, UT 84132, USA
5Center for Cognition and Sociality, Institute for Basic Science, Daejeon 305-811, Korea
6Co-first author
*Correspondence : Paul F. Worley
Summary
The immediate early gene Arc (also Arg3.1) produces rapid changes in synaptic properties that are linked to de novo translation. Here we develop a novel translation reporter that exploits the rapid maturation and “flash” kinetics of Gaussia luciferase (Gluc) to visualize Arc translation. Following glutamate stimulation, discrete Arc-Gluc bioluminescent flashes representing sites of de novo translation are detected within 15 s at distributed sites in dendrites, but not spines. Flashes are episodic, lasting ∼20 s, and may be unitary or repeated at ∼minute intervals at the same sites. Analysis of flash amplitudes suggests they represent the quantal product of one or more polyribosomes, while inter-flash intervals appear random, suggesting they arise from a stochastic process. Surprisingly, glutamate-induced translation is dependent on Arc open reading frame. Combined observations support a model in which stalled ribosomes are reactivated to rapidly generate Arc protein.
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