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조회 856  인쇄하기 주소복사 트위터 공유 페이스북 공유 
Global proteomic profiling of native outer membrane vesicles derived from Escherichia coli

Eun-Young Lee1, Joo Young Bang2, Gun Wook Park3, Dong-Sic Choi1, Ji Seoun Kang1, Hyun-Jung Kim1, Kyong-Su Park1, Jeong-Ok Lee1, Yoon-Keun Kim1, Kyung-Hoon Kwon3, Kwang-Pyo Kim2 and Yong Song Gho Professor1,*

1 Department of Life Science and Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea
2 Institute of Biomedical Science and Technology, Department of Molecular Biotechnology, Konkuk University, Seoul, Republic of Korea
3 Mass Spectrometer Development Team, Korea Basic Science Institute, Daejeon, Republic of Korea

*Corresponding author

Abstract
Gram-negative bacteria constitutively secrete native outer membrane vesicles (OMVs) into the extracellular milieu. Although recent progress in this area has revealed that OMVs are essential for bacterial survival and pathogenesis, the mechanism of vesicle formation and the biological roles of OMVs have not been clearly defined. Using a proteomics approach, we identified 141 protein components of Escherichia coli-derived native OMVs with high confidence; two separate analyses yielded identifications of 104 and 117 proteins, respectively, with 80 proteins overlapping between the two trials. In the group of identified proteins, the outer membrane proteins were highly enriched, whereas inner membrane proteins were lacking, suggesting that a specific sorting mechanism for vesicular proteins exists. We also identified proteins involved in vesicle formation, the removal of toxic compounds and attacking phage, and the elimination of competing organisms, as well as those involved in facilitating the transfer of genetic material and protein to other bacteria, targeting host cells, and modulating host immune responses. This study provides a global view of native bacterial OMVs. This information will help us not only to elucidate the biogenesis and functions of OMV from nonpathogenic and pathogenic bacteria but also to develop vaccines and antibiotics effective against pathogenic strains.

Keywords : E. coli DH5α; Microvesicles; Outer membrane vesicles; Tandem mass spectrometry; Vaccine

논문정보
- 형식: Research article
- 게재일: 2007년 09월 (BRIC 등록일 2016-06-08)
- 연구진: 국내연구진태극기
- 분야: Microbiology
- 피인용횟수: 120회 이상 인용된 논문
Dot/Icm 제 4 유형 커플링 단백질 복합체의 선택적 이펙터 단백질 인식[Nat. Commun.]
김현민
발표: 김현민 (KAIST)
일자: 2020년 9월 29일 (화) 오후 02시 (한국시간)
언어: 한국어
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