Junho K Hur1,6, Kyoungmi Kim1,6, Kyung Wook Been1,2, Gayoung Baek1, Sunghyeok Ye1,3, Junseok W Hur1,4, Seuk-Min Ryu1,2, Youn Su Lee1,5 & Jin-Soo Kim1,2
1Center for Genome Engineering, Institute for Basic Science, Seoul, Republic of Korea. 2Department of Chemistry, Seoul National University, Seoul, Republic of Korea. 3Basic Science, IBS School, Korea University of Science and Technology, Seoul, Republic of Korea. 4Department of Neurosurgery, Korea University College of Medicine, Seoul, Republic of Korea. 5ToolGen, Seoul, Republic of Korea. 6These authors contributed equally to this work.
Correspondence to : Jin-Soo Kim
To the Editor:
Cpf1 represents a class 2/type V CRISPR RNA-guided endonuclease1 that is distinct from the type II CRISPR-Cas9 nuclease, widely used for genome editing2, 3, 4, 5. It recognizes thymidine-rich protospacer-adjacent motif (PAM) sequences, expanding the range of RNA-guided genome editing beyond guanosine-rich PAM sequences recognized by Streptococcus pyogenes Cas9 (SpCas9). Yet, the potential of Cpf1 for targeted mutagenesis in whole organisms has not been demonstrated. Here, we report generation of mutant mice using preassembled recombinant CRISPR-Cpf1 ribonucleoproteins (RNPs).