한빛사 논문
Abstract
Jong-Eun Park,1,2,4 Hyerim Yi,1,2,4 Yoosik Kim,1,3,4 Hyeshik Chang,1,2 and V. Narry Kim1,2,*
1Center for RNA Research, Institute for Basic Science, Seoul 08826, Korea
2School of Biological Sciences, Seoul National University, Seoul 08826, Korea
3Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea
4Co-first author
*Correspondence: V. Narry Kim
SUMMARY
Poly(A) tails are critical for mRNA stability and translation. However, recent studies have challenged this view, showing that poly(A) tail length and translation efficiency are decoupled in non-embryonic cells. Using TAIL-seq and ribosome profiling, we investigate poly(A) tail dynamics and translational control in the somatic cell cycle. We find dramatic changes in poly(A) tail lengths of cell-cycle regulatory genes like CDK1, TOP2A, and FBXO5, explaining their translational repression in M phase. We also find that poly(A) tail length is coupled to translation when the poly(A) tail is <20 nucleotides. However, as most genes have >20 nucleotide poly(A) tails, their translation is regulated mainly via poly(A) tail lengthindependent mechanisms during the cell cycle. Specifically, we find that terminal oligopyrimidine (TOP) tract-containing transcripts escape global translational suppression in M phase and are actively translated. Our quantitative and comprehensive data provide a revised view of translational control in the somatic cell cycle.
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