Jung-Hwan Kim, PhDa, b, *, Eun-Ji Jeun, MSca, b, *, Chun-Pyo Hong, PhDa, b, Seong-Hoon Kim, MScd, Min Seong Jang, DVM, PhDa, b, Eun-Jung Lee, MSca, b, Sook Jin Moon, BSca, b, Chang Ho Yun, PhDa, Sin-Hyeog Im, PhDa, b, Seok-Geun Jeong, PhDc, Beom-Young Park, PhDc, Kyong-Tai Kim, PhDb, d, Ju-Young Seoh, MD, PhDe, Yoon-Keun Kim, MD, PhDf, Sung-Jong Oh, PhDg, Jun-Sang Ham, PhDc, , ‡, Bo-Gie Yang, PhDa, , ‡, Myoung Ho Jang, PhDa, , ‡
a Academy of Immunology and Microbiology, Institute for Basic Science, Pohang, Korea
b Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang, Korea
d Division of Molecular and Life Science, Department of Life Sciences, Pohang University of Science and Technology, Pohang, Korea
c National Institute of Animal Science, Rural Development Administration, Jeonju, Korea
e Department of Microbiology, Ewha Womans University Graduate School of Medicine, Seoul, Korea
f Department of Medicine, Ewha Womans University Graduate School of Medicine, Seoul, Korea
g Faculty of Biotechnology, College of Applied Life Science, Jeju National University, Jeju, Korea
* These authors contributed equally to this work.
‡ These authors contributed equally to this work.
Corresponding author: Myoung Ho Jang, PhD or Bo-Gie Yang, PhD, Jun Sang Ham, PhD
The incidence of food allergies has increased dramatically during the last decade. Recently, probiotics have been studied for the prevention and treatment of allergic disease.
We examined whether Bifidobacterium longum KACC 91563 and Enterococcus faecalis KACC 91532 have the capacity to suppress food allergies.
B longum KACC 91563 and E faecalis KACC 91532 were administered to BALB/c wild-type mice, in which food allergy was induced by using ovalbumin and alum. Food allergy symptoms and various immune responses were assessed.
B longum KACC 91563, but not E faecalis KACC 91532, alleviated food allergy symptoms. Extracellular vesicles of B longum KACC 91563 bound specifically to mast cells and induced apoptosis without affecting T-cell immune responses. Furthermore, injection of family 5 extracellular solute-binding protein, a main component of extracellular vesicles, into mice markedly reduced the occurrence of diarrhea in a mouse food allergy model.
B longum KACC 91563 induces apoptosis of mast cells specifically and alleviates food allergy symptoms. Accordingly, B longum KACC 91563 and family 5 extracellular solute-binding protein exhibit potential as therapeutic approaches for food allergies.
Key words : Food allergy; Bifidobacterium longum; probiotics; extracellular vesicle; mast cells; family 5 extracellular solute-binding protein; ovalbumin