Oh Seok Kwon†‡◇, Hyun Seok Song†§◇, Seon Joo Park†◇, Seung Hwan Lee†, Ji Hyun An†, Jin Wook Park†, Heehong Yang†, Hyeonseok Yoon∥, Joonwon Bae⊥, Tai Hyun Park*†#, and Jyongsik Jang*†
† School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Republic of Korea
‡ BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, Yuseong, Daejeon 305-600, Republic of Korea
§ Division of Bioconvergence Analysis, Korea Basic Science Institute (KBSI), Yuseong, Daejeon 169-148, Republic of Korea
∥School of Polymer Science and Engineering, and ?Department of Polymer Engineering, Graduate SchoolChonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 500-757, Republic of Korea
⊥ Department of Applied Chemistry, Dongduk Women’s University, Seongbuk-gu, Seoul, Republic of Korea
# Advanced Institutes of Convergence Technology, Suwon, Gyeonggi-do 443-270, Republic of Korea
◇O.S.K., H.S.S., and S.J.P. contributed equally to this work.
Human sensory-mimicking systems, such as electronic brains, tongues, skin, and ears, have been promoted for use in improving social welfare. However, no significant achievements have been made in mimicking the human nose due to the complexity of olfactory sensory neurons. Combinational coding of human olfactory receptors (hORs) is essential for odorant discrimination in mixtures, and the development of hOR-combined multiplexed systems has progressed slowly. Here, we report the first demonstration of an artificial multiplexed superbioelectronic nose (MSB-nose) that mimics the human olfactory sensory system, leading to high-performance odorant discriminatory ability in mixtures. Specifically, portable MSB-noses were constructed using highly uniform graphene micropatterns (GMs) that were conjugated with two different hORs, which were employed as transducers in a liquid-ion gated field-effect transistor (FET). Field-induced signals from the MSB-nose were monitored and provided high sensitivity and selectivity toward target odorants (minimum detectable level: 0.1 fM). More importantly, the potential of the MSB-nose as a tool to encode hOR combinations was demonstrated using principal component analysis.
Keywords: Multiplexed bioelectronic nose; graphene micropatterns; field-effect transistor; olfactory receptor; human mimicking; odorant discrimination