한빛사 논문
POSTECH
Dokyoung Kim †∥, Hyunsoo Moon †∥, Sung Hoon Baik ‡∥, Subhankar Singha †∥, Yong Woong Jun †, Taejun Wang §, Ki Hean Kim *§, Byung Sun Park ⊥, Junyang Jung ⊥, Inhee Mook-Jung *‡, and Kyo Han Ahn *†
† Department of Chemistry, Center for Electro-Photo Behaviors in Advanced Molecular Systems, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyungbuk 790-784, Republic of Korea
‡ Department of Biochemistry and Biomedical Sciences, College of Medicine, Seoul National University, 103 Daehak-Ro, Jongro-Gu, Seoul 110-799, Republic of Korea
§ Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyungbuk 790-784, Republic of Korea
⊥ Department of Anatomy and Neurobiology, School of Medicine, Biomedical Science Institute, Kyung Hee University, 26 Kyungheedae-Ro, Dongdaemun-Gu, Seoul 130-701, Republic of Korea
*Corresponding authors
∥These authors contributed equally.
Abstract
Fluorescence imaging of tissues offer an essential means for studying biological systems. Autofluorescence becomes a serious issue in tissue imaging under excitation at UV-vis wavelengths where biological molecules compete with the fluorophore. To address this critical issue, a novel class of fluorophores that can be excited at ∼900 nm under two-photon excitation conditions and emits in the red wavelength region (≥600 nm) has been disclosed. The new π-extended dipolar dye system shows several advantageous features including minimal autofluorescence in tissue imaging and pronounced solvent-sensitive emission behavior, compared with a widely used two-photon absorbing dye, acedan. As an important application of the new dye system, one of the dyes was developed into a fluorescent probe for amyloid-β plaques, a key biomarker of Alzheimer’s disease. The probe enabled in vivo imaging of amyloid-β plaques in a disease-model mouse, with negligible background signal. The new dye system has great potential for the development of other types of two-photon fluorescent probes and tags for imaging of tissues with minimal autofluorescence.
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