Yong-Soon Parka, 1, Swarnalee Duttaa, 1, Mina Anna, Jos M. Raaijmakersb, Kyungseok Parka
a Agricultural Microbiology Division, National Academy of Agricultural Science (NAAS), Rural Development Administration, Wanju 565-851, South Korea
b Department of Microbial Ecology, Netherlands Institute of Ecology, 6708 PB, Wageningen, The Netherlands
1 These authors contributed equally to this study
Corresponding author : Kyungseok Park
Volatile organic compounds (VOCs) from plant growth-promoting rhizobacteria (PGPR) play key roles in modulating plant growth and induced systemic resistance (ISR) to pathogens. Despite their significance, the physiological functions of the specific VOCs produced by Pseudomonas fluorescens SS101 (Pf.SS101) have not been precisely elucidated. The effects of Pf.SS101 and its VOCs on augmentation of plant growth promotion were investigated in vitro and in planta. A significant growth promotion was observed in plants exposed Pf.SS101 under both conditions, suggesting that its VOCs play a key role in promoting plant growth. Solid-phase micro-extraction (SPME) and a gas chromatography-mass spectrophotometer (GC-MS) system were used to characterize the VOCs emitted by Pf.SS101 and 11 different compounds were detected in samples inoculated this bacterium, including 13-Tetradecadien-1-ol, 2-butanone and 2-Methyl-n-1-tridecene. Application of these compounds resulted in enhanced plant growth. This study suggests that Pf.SS101 promotes the growth of plants via the release of VOCs including 13-Tetradecadien-1-ol, 2-butanone and 2-Methyl-n-1-tridecene, thus increasing understanding of the role of VOCs in plant-bacterial inter-communication.
Keywords : Pseudomonas fluorescens SS101; VOCs; GC-MS; 13-Tetradecadien-1-ol; Plant growth promotion