Howon Lee1,*, Hyoki Kim2,*, Sungsik Kim1,3, Taehoon Ry1,4, Hwangbeom Kim5, Duhee Bang6 & Sunghoon Kwon1,4,7,8
1 Institutes of Entrepreneurial BioConvergence, Seoul National University, Seoul 151-742, Republic of Korea. 2 Celemics Inc., 371-17, Gasan-dong, Geumcheongu, Seoul 153-718, Republic of Korea. 3 Interdisciplinary Program for Bioengineering, Seoul National University, Seoul 151-744, Republic of Korea. 4 Department of Electrical Engineering and Computer Science, Seoul National University, Seoul 151-744, Republic of Korea. 5 Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-1569, USA. 6 Department of Chemistry, Yonsei University, Seoul 120-749, Republic of Korea. 7 Center for Nanoparticle Research, Institute for Basic Science, Seoul National University, Seoul 151-742, Republic of Korea. 8 Seoul National University Hospital Biomedical Research Institute, 101 Daehakro, Chungrogu, Seoul National University Hospital, Seoul 110-744, Republic of Korea.
* These authors contributed equally to this work.
Correspondence to: Duhee Bang or Sunghoon Kwon
Writing DNA plays a significant role in the fields of synthetic biology, functional genomics and bioengineering. DNA clones on next-generation sequencing (NGS) platforms have the potential to be a rich and cost-effective source of sequence-verified DNAs as a precursor for DNA writing. However, it is still very challenging to retrieve target clonal DNA from high-density NGS platforms. Here we propose an enabling technology called ‘Sniper Cloning’ that enables the precise mapping of target clone features on NGS platforms and non-contact rapid retrieval of targets for the full utilization of DNA clones. By merging the three cutting-edge technologies of NGS, DNA microarray and our pulse laser retrieval system, Sniper Cloning is a week-long process that produces 5,188 error-free synthetic DNAs in a single run of NGS with a single microarray DNA pool. We believe that this technology has potential as a universal tool for DNA writing in biological sciences.