한빛사 논문
한국식품연구원, UST
Abstract
Lan-Sook Lee1,†, Ji Hea Choi1,2,†, Mi Jeong Sung1, Jin-Young hur1, Haeng Jeon Hur1, Jong-Dae Park1, Young-Chan Kim1, Eun-Ji Gu3, Byungjin Min4 and Hyun-Jin Kim3,*
1 Korea Food Research Institute, Bundang-gu, Seongnam, Gyeonggi, Republic of Korea
2 Department of Food Biotechnology, University of Science & Technology, Yuseong-gu, Daejeon, Republic of Korea
3 Division of Applied Life Sciences (BK21 plus), Department of Food Science & Technology, and Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Gyeongsang, Republic of Korea
4 Department of Food and Nutritional Sciences, College of Agriculture, Environment and Nutrition Sciences, Tuskegee University, Tuskegee, AL, USA
† These authors contributed equally to this work.
* Correspondence: Dr. Hyun-Jin Kim, Gyeongsang National University, 501 Jinjudaero, Jinju, Gyeongsang, Republic of Korea
Scope
: Green tea (GT) consumption helps to prevent and control obesity by stimulating hepatic lipid metabolism. However, GT-induced changes in serum and liver metabolomes associated with the anti-obesity effects are not clearly understood. The aim of this study was to identify and validate metabolomic profiles in the livers and sera of GT-fed obese mice to elucidate the relationship between GT consumption and obesity prevention.
Methods and results
: Serum and liver metabolites were analyzed in mice fed normal diet, high-fat diet (HFD), HFD with GT, and HFD with crude catechins, using liquid chromatography-quadrupole time-of-flight mass spectrometry. The addition of 1% GT to HFD reduced adipose tissue and the levels of blood triglycerides, glucose, insulin, and leptin elevated in HFD-fed mice. We proposed an HFD-induced obesity pathway and validated it by investigating the key regulatory enzymes of mitochondrial β-oxidation: carnitine palmitoyltransferase-1 and -2, acyl-coenzyme A dehydrogenase, and acetyl-coenzyme A acyltransferase. The results showed that HFD-induced abnormal mitochondrial β-oxidation was moderated by the consumption of caffeine- and theanine-enriched GT.
Conclusion
: Results of LC/MS-based metabolomic analysis of obese mice showed changes associated with abnormal lipid and energy metabolism, which were alleviated by GT intake, indicating the mechanism underlying the anti-obesity effects of GT.
Keywords: Acetyl-CoA acyltransferase 2; Fatty acid β-oxidation; Green tea; Metabolomics; Obesity
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