한빛사 논문
Abstract
Hyeong Cheol Park2, Man Lyang Kim, Yun Hwan Kang, Joo Mi Jeon, Jae Hyuk Yoo, Min Chul Kim, Chan Young Park, Jae Cheol Jeong, Byeong Cheol Moon, Ju Huck Lee, Hae Won Yoon, Sung-Ho Lee, Woo Sik Chung, Chae Oh Lim, Sang Yeol Lee, Jong Chan Hong* and Moo Je Cho*
Division of Applied Life Science (BK21 Program), Environmental Biotechnology Research Center and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea
*Corresponding authors
Abstract
The Ca2+-binding protein calmodulin mediates cellular Ca2+ signals in response to a wide array of stimuli in higher eukaryotes. Plants express numerous CaM isoforms. Transcription of one soybean (Glycine max) CaM isoform, SCaM-4, is dramatically induced within 30 min of pathogen or NaCl stresses. To characterize the cis-acting element(s) of this gene, we isolated an approximately 2-kb promoter sequence of the gene. Deletion analysis of the promoter revealed that a 130-bp region located between nucleotide positions -858 and -728 is required for the stressors to induce expression of SCaM-4. A hexameric DNA sequence within this region, GAAAAA (GT-1 cis-element), was identified as a core cis-acting element for the induction of the SCaM-4 gene. The GT-1 cis-element interacts with an Arabidopsis GT-1-like transcription factor, AtGT-3b, in vitro and in a yeast selection system. Transcription of AtGT-3b is also rapidly induced within 30 min after pathogen and NaCl treatment. These results suggest that an interaction between a GT-1 cis-element and a GT-1-like transcription factor plays a role in pathogen- and salt-induced SCaM-4 gene expression in both soybean and Arabidopsis.
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