한빛사 논문
Abstract
Hee Hun Yoon1,†, Suk Ho Bhang2,†, Taeho Kim1, Taekyung Yu3, Taeghwan Hyeon1 and Byung-Soo Kim1,*
1 School of Chemical and Biological Engineering, Seoul National Univeristy, Seoul, Republic of Korea
2 School of Chemical Engineering, Sungkyunkwan University, Suwon, Republic of Korea
3 Department of Chemical Engineering, Kyung Hee University, Yongin, Republic of Korea
†These authors contributed equally.
*Corresponding author
Abstract
Here, it is shown that graphene oxide (GO) can be utilized as both a cell-adhesion substrate and a growth factor protein-delivery carrier for the chondrogenic differentiation of adult stem cells. Conventionally, chondrogenic differentiation of stem cells is achieved by culturing cells in pellets and adding the protein transforming growth factor-β3 (TGF-β3), a chondrogenic factor, to the culture medium. However, pellets mainly provide cell-cell interaction and diffusional limitation of TGF-β3 may occur inside the pellet both of these factors may limit the chondrogenic differentiation of stem cells. In this study, GO sheets (size = 0.5-1 μm) were utilized to adsorb fibronectin (FN, a cell-adhesion protein) and TGF-β3 and were then incorporated in pellets of human adipose-derived stem cells (hASCs). The hybrid pellets of hASC-GO enhanced the chondrogenic differentiation of hASCs by adding the cell-FN interaction and supplying TGF-β3 effectively. This method may provide a new platform for stem cell culture for regenerative medicine.
Keywords: graphene oxide; chondrogenic differentiation; human adipose-derived stem cells; pellet culture; TGF-β3
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