Tae Sub Parka,b, Hong Jo Leea, Ki Hyun Kima, Jin-Soo Kimc, and Jae Yong Hana,1
aDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea;
bInstitute of Green-Bio Science and Technology, Seoul National University, Pyeongchang-gun, Gangwon-do 232-916, Korea; and
cDepartment of Chemistry, College of Natural Science, Seoul National University, Seoul 151-921, Korea
Genetically modified animals are used for industrial applications as well as scientific research, and studies on these animals contribute to a better understanding of biological mechanisms. Gene targeting techniques have been developed to edit specific gene loci in the genome, but the conventional strategy of homologous recombination with a gene-targeted vector has low efficiency and many technical complications. Here, we generated specific gene knockout chickens through the use of transcription activator-like effector nuclease (TALEN)-mediated gene targeting. In this study, we accomplished targeted knockout of the ovalbumin (OV) gene in the chicken primordial germ cells, and OV gene mutant offspring were generated through test-cross analysis. TALENs successfully induced nucleotide deletion mutations of ORF shifts, resulting in loss of chicken OV gene function. Our results demonstrate that the TALEN technique used in the chicken primordial germ cell line is a powerful strategy to create specific genome-edited chickens safely for practical applications.
genetic modification, programmable genome editing, germ-line chimera, poultry, egg protein
1To whom correspondence should be addressed.
Author contributions: T.S.P., J.-S.K., and J.Y.H. designed research; T.S.P., H.J.L., and K.H.K. performed research; T.S.P., H.J.L., and K.H.K. analyzed data; and T.S.P., J.-S.K., and J.Y.H. wrote the paper.