한빛사 논문
Su Ryon Shin1,2,8, Behnaz Aghaei-Ghareh-Bolagh1,2,3,†, Xiguang Gao4, Mehdi Nikkhah1,2,‡, Sung Mi Jung5, Alireza Dolatshahi-Pirouz1,2,6,8, Sang Bok Kim1,2, Sun Min Kim7, Mehmet R. Dokmeci1,2,8, Xiaowu (Shirley) Tang4,* and Ali Khademhosseini1,2,8,9,10,*
1 Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA
2 Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USA
3 Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden
4 Department of Chemistry & Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario, Canada
5 Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA
6 Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Aarhus, Denmark
7 Department of Mechanical Engineering, Inha University, Incheon, Republic of Korea
8 Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA
9 Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea
10 Department of Physics, King Abdulaziz University, Jeddah, Saudi Arabia
† Present Address: Charles Perkins Centre D17, The University of Sydney, NSW 2006, Australia
‡ Present Address: School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ, 85287, USA
*Corresponding author : Xiaowu (Shirley) Tang, Ali Khademhosseini
Abstract
Carbon-based nanomaterials have been considered promising candidates to mimic certain structure and function of native extracellular matrix materials for tissue engineering. Significant progress has been made in fabricating carbon nanoparticle-incorporated cell culture substrates, but only a limited number of studies have been reported on the development of 3D tissue constructs using these nanomaterials. Here, a novel approach to engineer 3D multilayer constructs using layer-by-layer (LbL) assembly of cells separated with self-assembled graphene oxide (GO)-based thin films is presented. The GO-based structures are shown to serve as cell adhesive sheets that effectively facilitate the formation of multilayer cell constructs with interlayer connectivity. By controlling the amount of GO deposited in forming the thin films, the thickness of the multilayer tissue constructs could be tuned with high cell viability. Specifically, this approach could be useful for creating dense and tightly connected cardiac tissues through the co-culture of cardiomyocytes and other cell types. In this work, the fabrication of stand-alone multilayer cardiac tissues with strong spontaneous beating behavior and programmable pumping properties is demonstrated. Therefore, this LbL-based cell construct fabrication approach, utilizing GO thin films formed directly on cell surfaces, has great potential in engineering 3D tissue structures with improved organization, electrophysiological function, and mechanical integrity.
Keywords: graphene oxide; poly-L-lysine; layer-by-layer; tissue constructs; cardiac tissue engineering
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