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최은별 (E. -B Choi)
POSTECH
조회 2078  인쇄하기 주소복사 트위터 공유 페이스북 공유 
Decreased diversity of nasal microbiota and their secreted extracellular vesicles in patients with chronic rhinosinusitis based on a metagenomic analysis

E.-B. Choi1, S.-W. Hong1, D.-K. Kim1, S. G. Jeon1, K.-R. Kim2, S.-H. Cho2, Y. S. Gho1, Y.-K. Jee3 & Y.-K. Kim1

1Department of Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang; 2Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Hanyang University, Seoul; 3Department of Internal Medicine, College of Medicine, Dankook University, Cheonan, Korea

Correspondence
Yoon-Keun Kim, Department of Life Sciences, Pohang University of Science and Technology (POSTECH), POSTECH BiotechCenter #351, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, Korea.
Seok-Hyun Cho, Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Hanyang University, 222-Wangshimniro, Seongdong-gu, Seoul 133-792, Korea.

Abstract

Background
Chronic rhinosinusitis (CRS) is an inflammatory process in the nasal cavity and paranasal sinuses, and bacteria have been considered to be a cause. Indeed, recent evidence indicates that bacteria-derived extracellular vesicles (EV) appear to be an important causative agent of inflammatory diseases. Here, we aimed to evaluate the diversity of nasal microbiota and their secreted EV in patients with CRS.

Methods
Nasal lavage (NAL) fluid samples were obtained from five patients with CRS with polyposis, three patients with CRS without polyposis, and three non-CRS controls. After preparation of bacteria and EV from samples using differential centrifugation, genomic DNA was extracted and 16S-rDNA amplicons were subjected to high-throughput pyrosequencing on a Roche 454 GS-FLX platform.

Results
Metagenomics showed that bacteria composition was positively correlated with EV composition. Samples from patients with CRS had greater bacterial abundance and lower diversity, both from bacteria and the EV portion of samples, compared with non-CRS samples. At each phylogenetic level, Bacteroidetes decreased while Proteobacteria increased in the CRS group at the phylum level. At the genus level, Prevotella spp. decreased in the CRS group, while Staphylococcus spp. increased from both bacteria and EV. Moreover, Staphylococcus aureus and its secreting EV compositions were higher in samples from CRS with polyps compared with CRS without polyps.

Conclusions
These results suggest that patients with CRS have altered nasal microbiota and decreased diversity in bacterial compositions as well as increased S. aureus abundance in those patients with polyps.

Keywords: chronic rhinosinusitis; extracellular vesicles; metagenomic analysis; microbial diversity

논문정보 F1000선정
- 형식: Research article
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- 연구진: 국내연구진태극기
- 분야: Medicine
- 추천: Faculty of 1000 Biology
- 추천사유: F1000Prime Recommendations, Dissents and Comments for [Choi EB et al., Allergy 2014, 69(4):517-26]. In F1000Prime, 09 Jul 2014; F1000Prime.com/718306562
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