한빛사 논문
Abstract
Ju Hee Ryua, b, Il-Kwon Kimc, e, Seung-Woo Choa, b, Myeong-Chan Chod, Kyung-Kuk Hwangd, Hainan Piaod, Shuguang Piaod, Sang Hyun Lime, Yoo Sun Honge, Cha Yong Choib, Kyung Jong Yooe, Byung-Soo Kima
a Department of Chemical Engineering, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul 133-791, South Korea
b School of Chemical Engineering, Seoul National University, Seoul 151-742, South Korea
c Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, South Korea
d Department of Cardiology, College of Medicine, Chungbuk National University, South Korea
e Division of Cardiovascular Surgery, Yonsei Cardiovascular Hospital and Research Institute, Yonsei University College of Medicine, Seoul 120-752, South Korea
Corresponding authors : Kyung Jong Yoo, Byung-Soo Kim
Abstract
Neovascularization may improve cardiac function and prevent further scar tissue formation in infarcted myocardium. A number of studies have demonstrated that bone marrow-derived cells have the potential to induce neovascularization in ischemic tissues. In this study, we hypothesized that implantation of bone marrow mononuclear cells (BMMNCs) using injectable fibrin matrix further enhances neovascularization in infarcted myocardium compared to BMMNC implantation without matrix. To test this hypothesis, infarction was induced in rat myocardium by cryoinjury. Three weeks later, rat BMMNCs were mixed with fibrin matrix and injected into the infarcted myocardium. Injection of either BMMNCs or medium alone into infarcted myocardium served as controls. Eight weeks after the treatments, histological analyses indicated that implantation of BMMNCs using fibrin matrix resulted in more extensive tissue regeneration in the infarcted myocardium compared to BMMNC implantation without matrix. Examination with fluorescence microscopy revealed that cells labeled with a fluorescent dye prior to implantation survived in the infarcted myocardium at 8 weeks of implantation. Importantly, implantation of BMMNCs using fibrin matrix resulted in much more extensive neovascularization in infarcted myocardium than BMMNC implantation without matrix. The microvessel density in infarcted myocardium was significantly higher (p<0.05) when BMMNCs were implanted using fibrin matrix (350±22 microvessels/mm2) compared to BMMNC implantation without matrix (262±13 microvessels/mm2) and medium injection (76±9 microvessels/mm2). In addition, average internal diameter of microvessels was significantly larger (p<0.05) in BMMNC implantation with fibrin matrix group (14.6±1.2 μm) than BMMNC implantation without matrix group (10.2±0.7 μm) and medium injection group (7.3±0.5 μm). These results suggest that fibrin matrix could serve as a cell implantation matrix that enhances neovascularization efficacy for myocardial infarction treatment.
Keywords : Fibrin marix; Bone marrow mononuclear cells; Myocardial infarction; Neovascularization
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