[DEBUG-WINDOW 처리영역 보기]
즐겨찾기  |  뉴스레터  |  오늘의 정보  |  e브릭몰e브릭몰 회원가입   로그인
BRIC홈 한국을 빛내는 사람들
라이카써머캠프
스폰서배너광고 안내  배너1 배너2 배너3 배너4
과학으로 본 코로나19 (COVID-19)
전체보기 한빛사논문 상위피인용논문 인터뷰 그이후 한빛사통계
조남혁
조남혁 (Nam-Hyuk Cho) 저자 이메일 보기
서울대학교 의과대학
조회 2254  인쇄하기 주소복사 트위터 공유 페이스북 공유 
Orientia tsutsugamushi Subverts Dendritic Cell Functions by Escaping from Autophagy and Impairing Their Migration

Ji-Hye Choi1, Taek-Chin Cheong1, Na-Young Ha1, Youngho Ko1, Chung-Hyun Cho2, Ju-Hong Jeon3, Insuk So3, In-Kyu Kim4, Myung-Sik Choi1, Ik-Sang Kim1, Nam-Hyuk Cho1,5*

1 Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul, Republic of Korea, 2 Pharmacology, Seoul National University College of Medicine, Seoul, Republic of Korea, 3 Physiology, Seoul National University College of Medicine, Seoul, Republic of Korea, 4 Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, Republic of Korea, 5 Institute of Endemic Disease, Seoul National University Medical Research Center and Bundang Hospital, Jongno-Gu, Seoul, Republic of Korea

Abstract

Background
Dendritic cells (DCs) are the most potent antigen-presenting cells that link innate and adaptive immune responses, playing a pivotal role in triggering antigen-specific immunity. Antigen uptake by DCs induces maturational changes that include increased surface expression of major histocompatibility complex (MHC) and costimulatory molecules. In addition, DCs actively migrate to regional lymph nodes and activate antigen-specific naive T cells after capturing antigens. We characterize the functional changes of DCs infected with Orientia tsutsugamushi, the causative agent of scrub typhus, since there is limited knowledge of the role played by DCs in O. tsutsugamushi infection.

Methodology/Principal Finding
O. tsutsugamushi efficiently infected bone marrow-derived DCs and induced surface expression of MHC II and costimulatory molecules. In addition, O. tsutsugamushi induced autophagy activation, but actively escaped from this innate defense system. Infected DCs also secreted cytokines and chemokines such as IL-6, IL-12, MCP5, MIP-1α, and RANTES. Furthermore, in vitro migration of DCs in the presence of a CCL19 gradient within a 3D collagen matrix was drastically impaired when infected with O. tsutsugamushi. The infected cells migrated much less efficiently into lymphatic vessels of ear dermis ex vivo when compared to LPS-stimulated DCs. In vivo migration of O. tsutsugamushi-infected DCs to regional lymph nodes was significantly impaired and similar to that of immature DCs. Finally, we found that MAP kinases involved in chemotactic signaling were differentially activated in O. tsutsugamushi-infected DCs.

Conclusion/Significance
These results suggest that O. tsutsugamushi can target DCs to exploit these sentinel cells as replication reservoirs and delay or impair the functional maturation of DCs during the bacterial infection in mammals.

논문정보 F1000선정
- 형식: Research article
- 게재일: 2013년 01월 (BRIC 등록일 2014-05-30)
- 연구진: 국내연구진태극기
- 분야: Parasitology
- 추천: Faculty of 1000 Biology
- 추천사유: F1000Prime Recommendations, Dissents and Comments for [Choi JH et al., PLoS Negl Trop Dis 2013, 7(1):e1981]. In F1000Prime, 26 May 2014; F1000Prime.com/718402458
광유전학의 과거, 현재와 미래[Neuron]
김윤석
발표: 김윤석 (Stanford University)
일자: 2020년 7월 30일 (목) 오후 02시 (한국시간)
언어: 한국어
참석자 접수신청하기

  댓글 0
등록
 
목록
IDT Korea
관련링크
조남혁 님 전체논문보기 >
관련인물
관련분야 논문보기
Parasitology

외부링크
Google (by Nam-Hyuk Cho)
Pubmed (by Nam-Hyuk Cho)
제품평가
[비아이코퍼레이션]
e브릭몰(연구용 제품 온라인 구매 사이트)
제품이미지
모집인원: 50명
모집기간: ~7/15
신청조건: BRIC 회원
평가자 혜택

- 참가자 전원 3만원 상품권 제공

제품평가자 모집중
위로가기
한빛사 홈  |  한빛사FAQ  |  한빛사 문의 및 제안
 |  BRIC소개  |  이용안내  |  이용약관  |  개인정보처리방침  |  이메일무단수집거부
Copyright © BRIC. All rights reserved.  |  문의 member@ibric.org
트위터 트위터    페이스북 페이스북   유튜브 유튜브    RSS서비스 RSS
머크