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박재형 (Jae Hyung Park) 저자 이메일 보기
성균관대학교
 
조회 3041  인쇄하기 주소복사 트위터 공유 페이스북 공유 
Inhibition of Notch signalling ameliorates experimental inflammatory arthritis

Jong-Sung Park1, Seol-Hee Kim1,2, Kwangmeyung Kim3, Cheng-Hao Jin4, Ki Young Choi5, Jiyeon Jang1, Yuri Choi1, A-Ryeong Gwon1, Sang-Ha Baik1, Ui Jeong Yun1, Su Young Chae1, Seulki Lee6, Young Mo Kang7, Kang Choon Lee1, Thiruma V Arumugam1,8, Mark P Mattson9,10, Jae Hyung Park2, Dong-Gyu Jo1

1School of Pharmacy, Sungkyunkwan University, Suwon, Korea
2Departments of Polymer Science and Chemical Engineering, Sungkyunkwan University, Suwon, Korea
3Biomedical Research Center, Korea Institute of Science and Technology, Seoul, Korea
4Department of Biochemistry and Molecular Biology, College of Life Science & Technology, Heilongjiang Bayi Agricultural University, Daqing, China
5Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA
6Russell H. Morgan Department of Radiology and Radiological Science, Center for Cancer Nanotechnology Excellence, Center for Nanomedicine at the Wilmer Eye Institute, Johns Hopkins University, Baltimore, Maryland, USA
7Department of Internal Medicine (Rheumatology), Kyungpook National University School of Medicine, Daegu, Korea
8Department of Physiology, Yong Loo Lin School Medicine, National University of Singapore, Singapore, Singapore
9Laboratory of Neurosciences, National Institute on Aging Intramural Research Program, Baltimore, Maryland, USA
10Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Correspondence to Professor Dong-Gyu Jo, School of Pharmacy, Sungkyunkwan University, 300 Cheoncheon-Dong, Suwon 440-746, Korea, or Professor Jae Hyung Park, Departments of Polymer Science and Chemical Engineering, Sungkyunkwan University, 300 Cheoncheon-Dong, Suwon 440-746, Korea.

Abstract

Objective To test the hypothesis that Notch signalling plays a role in the pathogenesis of rheumatoid arthritis (RA) and to determine whether pharmacological inhibition of Notch signalling with γ-secretase inhibitors can ameliorate the RA disease process in an animal model.

Methods Collagen-induced arthritis was induced in C57BL/6 or Notch antisense transgenic mice by immunisation with chicken type II collagen (CII). C57BL/6 mice were administered with different doses of inhibitors of γ-secretase, an enzyme required for Notch activation, at disease onset or after onset of symptoms. Severity of arthritis was monitored by clinical and histological scores, and in vivo non-invasive near-infrared fluorescence (NIRF) images. Micro-CT was used to confirm joint destruction. The levels of CII antibodies and cytokines in serum were determined by ELISA and bead-based cytokine assay. The expression levels of cytokines were studied by quantitative PCR in rheumatoid synovial fibroblasts.

Results The data show that Notch signalling stimulates synoviocytes and accelerates their production of proinflammatory cytokines and immune responses involving the upregulation of IgG1 and IgG2a. Pharmacological inhibition of γ-secretase and antisense-mediated knockdown of Notch attenuates the severity of inflammatory arthritis, including arthritis indices, paw thickness, tissue damage and neutrophil infiltration, and reduces the levels of active NF-κB, ICAM-1, proinflammatory cytokines and matrix metalloproteinase-3 activity in the mouse model of RA.

Conclusions These results suggest that Notch is involved in the pathogenesis of RA and that inhibition of Notch signalling is a novel approach for treating RA.

논문정보 F1000선정
- 형식: Research article
- 게재일: 2013년 11월 (BRIC 등록일 2013-12-19)
- 연구진: 국내(교신)+국외 연구진태극기
- 추천: Faculty of 1000 Biology
- 추천사유: F1000Prime Recommendations, Dissents and Comments for [Park JS et al., Ann Rheum Dis 2013]. In F1000Prime, 19 Dec 2013; F1000Prime.com/718181581
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