한빛사 논문
Abstract
S Chul Kwon1,2, Hyerim Yi1,2, Katrin Eichelbaum3, Sophia Fohr3, Bernd Fischer3, Kwon Tae You1,2, Alfredo Castello3, Jeroen Krijgsveld3, Matthias W Hentze3,4 & V Narry Kim1,2,*
1Center for RNA Research, Institute for Basic Science (IBS), Seoul, Korea. 2School of Biological Sciences, Seoul National University, Seoul, Korea. 3European Molecular Biology Laboratory (EMBL), Heidelberg, Germany. 4Molecular Genetics Division, Victor Chang Cardiac Research Institute, Sydney, Australia.
*Correspondence should be addressed to V.N.K
RNA-binding proteins (RBPs) have essential roles in RNA-mediated gene regulation, and yet annotation of RBPs is limited mainly to those with known RNA-binding domains. To systematically identify the RBPs of embryonic stem cells (ESCs), we here employ interactome capture, which combines UV cross-linking of RBP to RNA in living cells, oligo(dT) capture and MS. From mouse ESCs (mESCs), we have defined 555 proteins constituting the mESC mRNA interactome, including 283 proteins not previously annotated as RBPs. Of these, 68 new RBP candidates are highly expressed in ESCs compared to differentiated cells, implicating a role in stem-cell physiology. Two well-known E3 ubiquitin ligases, Trim25 (also called Efp) and Trim71 (also called Lin41), are validated as RBPs, revealing a potential link between RNA biology and protein-modification pathways. Our study confirms and expands the atlas of RBPs, providing a useful resource for the study of the RNA-RBP network in stem cells.
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