Jaehwan Jeong¹, Namjin Cho¹, Daehee Jung, Duhee Bang^*

Department of Chemistry, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Korea

^*Corresponding author : Duhee Bang

¹These authors contributed equally to this work.

Abstract
Genome engineering has been developed to create useful strains for biological studies and industrial uses. However, a continuous challenge remained in the field: technical limitations in high-throughput screening and precise manipulation of strains. Today, technical improvements have made genome engineering more rapid and efficient. This review introduces recent advances in genome engineering technologies applied to Escherichia coli, as well as Multiplex Automated Genome Engineering (MAGE), a recent technique proposed as a powerful toolkit due to its straightforward process, rapid experimental procedures, and highly efficient properties.

Keywords : Genome engineering; λ red recombination; Multiplex automated genome engineering