한빛사 논문
Abstract
Samir Ben Chaabane1, Renyi Liu2, Viswanathan Chinnusamy2,3, Yerim Kwon4, Joo-hyuk Park4, Seo Yeon Kim5, Jian-Kang Zhu2,6,7, Seong Wook Yang1,* and Byeong-ha Lee4,*
1Department of Plant Biology and Biotechnology, Faculty of Life Science, University of Copenhagen, Thovanlsensvej 40, 1871 Frederiksberg, Copenhagen, Denmark, 2Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA, 3Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi 110012, India, 4Department of Life Science, Sogang University, Seoul 121-742, Korea, 5Department of Life Science, Ewha Womans University, Seoul 120-750, Korea, 6Department of Horticulture and Landscape Architecture, University of Purdue, West Lafayette, IN 47907, USA and 7Shanghai Center for Plant Stress Biology and Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai, People’s Republic of China
*To whom correspondence should be addressed.
Abstract
MicroRNAs (miRNAs) are small regulatory RNAs that have important regulatory roles in numerous developmental and metabolic processes in most eukaryotes. In Arabidopsis, DICER-LIKE1 (DCL1), HYPONASTIC LEAVES 1, SERRATE, HUA ENHANCER1 and HASTY are involved in processing of primary miRNAs (pri-miRNAs) to yield precursor miRNAs (pre-miRNAs) and eventually miRNAs. In addition to these components, mRNA cap-binding proteins, CBP80/ABA HYPERSENSITIVE1 and CBP20, also participate in miRNA biogenesis. Here, we show that STABILIZED1 (STA1), an Arabidopsis pre-mRNA processing factor 6 homolog, is also involved in the biogenesis of miRNAs. Similar to other miRNA biogenesis-defective mutants, sta1-1 accumulated significantly lower levels of mature miRNAs and concurrently higher levels of pri-miRNAs than wild type. The dramatic reductions of mature miRNAs were associated with the accumulation of their target gene transcripts and developmental defects. Furthermore, sta1-1 impaired splicing of intron containing pri-miRNAs and decreased transcript levels of DCL1. These results suggest that STA1 is involved in miRNA biogenesis directly by functioning in pri-miRNA splicing and indirectly by modulating the DCL1 transcript level.
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