Seung Woo Cho1.3, Sojung Kim1.3, Jong Min Kim1,2 & Jin-Soo Kim1,2,*
1National Creative Research Initiatives Center for Genome Engineering, Seoul National University, Seoul, South Korea. 2Department of Chemistry, Seoul National University, Seoul, South Korea. 3These authors contributed equally to this work.
*Correspondence to: Jin-Soo Kim
We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33%.