Young Hoon Sung1,6, In-Jeoung Baek2,6, Duk Hyoung Kim3,6, Jisun Jeon1, Jaehoon Lee1, Kyunghee Lee4, Daewon Jeong4, Jin-Soo Kim3,* & Han-Woong Lee1,5,*
1Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul, Republic of Korea. 2Asan Institute for Life Sciences, University of Ulsan College of Medicine, Seoul, Republic of Korea. 3Department of Chemistry, Seoul National University, Seoul, Republic of Korea. 4Department of Microbiology and Aging-Associated Vascular Disease Research Center, Yeungnam University, Daegu, Republic of Korea. 5Laboratory Animal Research Center, Yonsei University, Seoul, Republic of Korea. 6These authors contributed equally to this work.
*Correspondence to: Han-Woong Lee or Jin-Soo Kim
Phenotypic analysis of gene-specific knockout mice has transformed our understanding of in vivo gene functions. Generation of knockout mice, however, remains a time-consuming and expensive process. Transcription activator-like (TAL) effector nucleases (TALENs) are highly effective in inducing mutations at specific genomic loci1, 2, and consequently TALEN-mediated mutagenesis in zygotes is a potential alternative to conventional gene targeting in mice. However, to the best of our knowledge, gene knockout mice have yet to be created using TALENs. Here, we report the generation of mice with a genetic knockout of the progesterone immunomodulatory binding factor 1(Pibf1) or selenoprotein W, muscle 1 (Sepw1) gene using TALENs...