한빛사 논문
Abstract
Yu-Ran Na†,⊥, So Yeon Kim †,⊥, Jellert T. Gaublomme ‡, Alex K. Shalek ‡, Marsela Jorgolli §, Hongkun Park ‡§, and Eun Gyeong Yang *†
† Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, South Korea
‡ Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, United States
§ Department of Physics, Harvard University, 17 Oxford Street, Cambridge, Massachusetts 02138, United States
⊥Author Contributions
These authors contributed equally.
*Corresponding Author
Abstract
Developing a detailed understanding of enzyme function in the context of an intracellular signal transduction pathway requires minimally invasive methods for probing enzyme activity in situ. Here, we describe a new method for monitoring enzyme activity in living cells by sandwiching live cells between two vertical silicon nanowire (NW) arrays. Specifically, we use the first NW array to immobilize the cells and then present enzymatic substrates intracellularly via the second NW array by utilizing the NWs’ ability to penetrate cellular membranes without affecting cells’ viability or function. This strategy, when coupled with fluorescence microscopy and mass spectrometry, enables intracellular examination of protease, phosphatase, and protein kinase activities, demonstrating the assay’s potential in uncovering the physiological roles of various enzymes.
Keywords : Silicon nanowire; sandwich assembly; live cell assay; enzyme activity
논문정보
관련 링크
연구자 키워드
연구자 ID
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기