한빛사 논문
Abstract
Hye Ran Koha,b, Mary Anne Kidwellc, Kaushik Ragunathand, Jennifer A. Doudnac,e,f, and Sua Myongb,d,g,1
aDepartment of Physics, University of Illinois, Urbana, IL 61801;
bInstitute for Genomic Biology, Urbana, IL 61801;
cDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720;
dDepartment of Biophysics and Computational Biology, University of Illinois, Urbana, IL61801;
eHoward Hughes Medical Institute, University of California, Berkeley, CA 94720;
fPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720; and
gDepartment of Bioengineering, University of Illinois, Urbana, IL 61801
Edited by Brenda L. Bass, University of Utah, Salt Lake City, UT, and accepted by the Editorial Board November 15, 2012 (received for review July 27, 2012)
Abstract
The proteins harboring double-stranded RNA binding domains (dsRBDs) play diverse functional roles such as RNA localization, splicing, editing, export, and translation, yet mechanistic basis and functional significance of dsRBDs remain unclear. To unravel this enigma, we investigated transactivation response RNA binding protein (TRBP) consisting of three dsRBDs, which functions in HIV replication, protein kinase R(PKR)?mediated immune response, and RNA silencing. Here we report an ATP-independent diffusion activity of TRBP exclusively on dsRNA in a length-dependent manner. The first two dsRBDs of TRBP are essential for diffusion, whereas the third dsRBD is dispensable. Two homologs of TRBP, PKR activator and R3D1-L, displayed the same diffusion, implying a universality of the diffusion activity among this protein family. Furthermore, a Dicer?TRBP complex on dsRNA exhibited dynamic diffusion, which was correlated with Dicer’s catalytic activity. These results implicate the dsRNA-specific diffusion activity of TRBP that contributes to enhancing siRNA and miRNA processing by Dicer.
RNA interference, single molecule FRET
1To whom correspondence should be addressed.
Author contributions: H.R.K., M.A.K., J.A.D., and S.M. designed research; H.R.K. and M.A.K. performed research; K.R. contributed new reagents/analytic tools; H.R.K. analyzed data; and H.R.K., J.A.D., and S.M. wrote the paper.
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