Donghun Kim, Nam Hee Kim, Dr. Sun Hee Kim*
Division of Materials Science, Korea Basic Science Institute, Daejeon, 305-333 (Korea)
*To whom correspondence may be addressed.
The copper(II) ion coordination mode of CuII-Aβ(1-16) at physiological pH was characterized by 34 GHz pulsed ENDOR spectroscopy. 1H/2H ENDOR and 14N ENDOR demonstrate the NH2 terminus of aspartic acid is an equatorial ligand of CuII, and 17O ENDOR together with 1H ENDOR suggest that water is an axial ligand (see picture; Cu pink, O red, N blue).
This work was supported by KBSI grant (T32411) and Seoul R&BD program (ST110029M0212351) to S.H.K. We gratefully appreciate Dr. Sunwoo Kang for his help with DFT calculations, and Prof. Brian M. Hoffman and Dr. Peter Doan at Northwestern University for providing the ENDOR simulation code.
Keywords: Alzheimer’s disease; amyloid β peptide; copper; ENDOR spectroscopy; EPR