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DJ-1 regulates mast cell activation and IgE-mediated allergic responses

Do Kyun Kim, PhDa,*, Hyuk Soon Kim, MSa,*, A-Ram Kim, MSa, Ji Hyung Kim, BSa, Bokyung Kim, PhDa, Geunwoong Noh, MD, PhDb, Hyung Sik Kim, PhDc, Michael A. Beaven, PhDd, Young Mi Kim, PhDe, Wahn Soo Choi, PhDa

aDepartment of Immunology and Physiology, Functional Genomics Institute, College of Medicine, Konkuk University, Chungju, Korea
bSubdivision of Allergy and Clinical Immunology, Department of Pediatrics, Chungnam National University Hospital, Daejeon, Korea
cCollege of Pharmacy, Pusan National University, Busan, Korea
dLaboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Md
eCollege of Pharmacy, Duksung Women’s University, Seoul, Korea

Corresponding author: Wahn Soo Choi, PhD, Department of Immunology, College of Medicine, Konkuk University, Chungju 380-701, Korea.

*These authors contributed equally to this work.

DJ-1 is an antioxidant protein known to reduce levels of reactive oxygen species (ROS), but its presence or function in mast cells and allergic diseases is unknown.

We sought to determine the role and mechanism of DJ-1 in allergic responses in vitro and in vivo.

ROS and DJ-1 levels in serum or culture medium were measured with ELISA kits. The role of DJ-1 was evaluated in mast cell cultures and passive cutaneous anaphylaxis in normal or DJ-1 knockout (KO) mice. The mechanism of DJ-1 action was examined by using immunoblotting, immunoprecipitation, RT-PCR, and other molecular biological approaches.

Patients with atopic dermatitis had increased levels of ROS and diminished levels of DJ-1. DJ-1 KO mice exhibited enhanced passive cutaneous anaphylaxis and augmented ROS levels in sera and bone marrow-derived mast cells (BMMCs). Furthermore, antigen-induced degranulation and production of TNF-α and IL-4 were significantly amplified in DJ-1 KO and anti-DJ-1 small interfering RNA-transfected BMMCs compared with that seen in wild-type (WT) BMMCs. Studies with these cells and BMMCs transfected with small interfering RNAs against the phosphatases Src homology domain 2-containing protein tyrosine phosphatase (SHP) 1 and SHP-2 revealed that the DJ-1 KO phenotype could be attributed to suppression of SHP-1 activity and enhancement of SHP-2 activity, leading to strengthened signaling through linker for activation of T cells, phospholipase Cγ, and mitogen-activated protein kinases.

A deficiency or constitutive activation of DJ-1 can have implications in mast cell-driven allergic diseases, such as asthma and anaphylaxis.

Key words: DJ-1, reactive oxygen species, mast cells, allergy, FcεRI-mediated signals

- 형식: Research article
- 게재일: 2012년 11월 (BRIC 등록일 2012-11-29)
- 연구진: 국내(교신)+국외 연구진태극기
- 분야: Immunology
광유전학의 과거, 현재와 미래[Neuron]
발표: 김윤석 (Stanford University)
일자: 2020년 7월 30일 (목) 오후 02시 (한국시간)
언어: 한국어
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