한빛사 논문
Abstract
Indong Jun1, Seok Joo Kim1, Ji-Hye Lee1, Young Jun Lee1, Young Min Shin1, Eunpyo Choi2, Kyung Min Park3, Jungyul Park2, Ki Dong Park3, Heungsoo Shin1,*
1 Department of Bioengineering, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul 133-791, Republic of Korea
2 Department of Mechanical Engineering, Sogang University, Sinsu-dong, Mapo-gu, Seoul 121-742, Republic of Korea
3 Department of Molecular Science and Technology, Ajou University, 5 WonChun-dong, Yeoungtong-gu, Suwon 443-749, Republic of Korea
*To whom correspondence may be addressed.
Abstract
The structure of tissue plays a critical role in its function and therefore a great deal of attention has been focused on engineering native tissue-like constructs for tissue engineering applications. Transfer printing of cell layers is a new technology that allows controlled transfer of cell layers cultured on smart substrates with defined shape and size onto tissue-specific defect sites. Here, the temperature-responsive swelling-deswelling of the hydrogels with groove patterns and their versatile and simple use as a template to harvest cell layers with anisotropic extracellular matrix assembly is reported. The hydrogels with a cell-interactive peptide and anisotropic groove patterns are obtained via enzymatic polymerization. The results show that the cell layer with patterns can be easily transferred to new substrates by lowering the temperature. In addition, multiple cell layers are stacked on the new substrate in a hierarchical manner and the cell layer is easily transplanted onto a subcutaneous region. These results indicate that the evaluated hydrogel can be used as a novel substrate for transfer printing of artificial tissue constructs with controlled structural integrity, which may hold potential to engineer tissue that can closely mimic native tissue architecture.
Keywords: biomimetics; hydrogels; stimuli-responsive materials; tissue engineering
논문정보
관련 링크
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기