한빛사 논문
Abstract
Yong-Seok Leea,1, Sun-Lim Choia,1, Heejung Juna,1, Se-Jeong Yima, Jin-A Leeb, Hyoung F. Kima, Seung-Hee Leea, Jaehoon Shima, Kyungmin Leec, Deok-Jin Jangd, and Bong-Kiun Kaanga,e,2
aNational Creative Research Initiative Center for Memory, Departments of Biological Sciences and
eBrain and Cognitive Sciences, College of Natural Sciences, Seoul National University, Seoul 151-747, Korea;
bDepartment of Biotechnology, College of Life Science and Nano Technology, Hannam University, Daejeon 305-811, Korea;
cDepartment of Anatomy, Graduate School of Medicine, Brain Science and Engineering Institute, Kyungpook National University, Daegu 700-422, Korea; and
dDepartment of Applied Biology, College of Ecology and Environment, Kyungpook National University, Sangju 742-711, Korea
Abstract
The consolidation of long-term memory for sensitization and synaptic facilitation in Aplysia requires synthesis of new mRNA including the immediate early gene Aplysia CCAAT enhancer-binding protein (ApC/EBP). After the rapid induction of ApC/EBP expression in response to repeated treatments of 5-hydroxytryptamine (5-HT), ApC/EBP mRNA is temporarily expressed in sensory neurons of sensory-to-motor synapses. However, the molecular mechanism underlying the rapid degradation of ApC/EBP transcript is not known. Here, we cloned an AU-rich element (ARE)-binding protein, ApAUF1, which functions as a destabilizing factor for ApC/EBP mRNA. ApAUF1 was found to bind to the 3′ UTR of ApC/EBP mRNA that contains AREs and subsequently reduces the expression of ApC/EBP 3′ UTR-containing reporter genes. Moreover, overexpression of ApAUF1 inhibited the induction of ApC/EBP mRNA in sensory neurons and also impaired long-term facilitation of sensory-to-motor synapses by repetitive 5-HT treatments. These results provide evidence for a critical role of the posttranscriptional modification of ApC/EBP mRNA during the consolidation of synaptic plasticity.
Footnotes
1Y.-S.L., S.-L.C., and H.J. contributed equally to this work.
2To whom correspondence should be addressed.
Author contributions: Y.-S.L., S.-L.C., H.J., and B.-K.K. designed research; Y.-S.L., S.-L.C., H.J., S.-J.Y., J.-A.L., H.F.K., S.-H.L., J.S., K.L., and D.-J.J. performed research; Y.-S.L., S.-L.C., H.J., J.-A.L., and H.F.K. analyzed data; and Y.-S.L. and B.-K.K. wrote the paper.
논문정보
관련 링크
연구자 키워드
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기