[DEBUG-WINDOW 처리영역 보기]
즐겨찾기  |  뉴스레터  |  오늘의 정보  |  e브릭몰e브릭몰 sale 회원가입   로그인
BRIC홈 한국을 빛내는 사람들
검색광고안내
과학기술용어에 대한 과기인 인식조사
스폰서배너광고 안내  배너1 배너2 배너3 배너4
과학으로 본 코로나19 (COVID-19)
전체보기 한빛사논문 상위피인용논문 인터뷰 그이후 한빛사통계
김천생
김천생 (Chonsaeng Kim) 저자 이메일 보기
Children’s Hospital of Philadelphia
저자CV 보기
190 KB
 
조회 2696  인쇄하기 주소복사 트위터 공유 페이스북 공유 
Echovirus 7 Entry into Polarized Intestinal Epithelial Cells Requires Clathrin and Rab7

Chonsaeng Kima and Jeffrey M. Bergelsona,b

Division of Infectious Diseases, Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA,a and
Department of Pediatrics, University of Pennsylvania, Philadelphia, Pennsylvania, USAb

Address correspondence to Jeffrey M. Bergelson

Editor Peter Palese, Mount Sinai School of Medicine

ABSTRACT
Enteroviruses invade the host by crossing the intestinal mucosa, which is lined by polarized epithelium. A number of enteroviruses, including echoviruses (EV) and group B coxsackieviruses (CVB), initiate infection by attaching to decay-accelerating factor (DAF), a molecule that is highly expressed on the apical surface of polarized epithelial cells. We previously observed that entry of DAF-binding CVB3 into polarized intestinal epithelial cells occurs by an unusual endocytic mechanism that requires caveolin but does not involve clathrin or dynamin. Here we examined the entry of a DAF-binding echovirus, EV7. We found that drugs, small interfering RNAs (siRNAs), and dominant negative mutants that target factors required for clathrin-mediated endocytosis, including clathrin and dynamin, inhibited both EV7 infection and internalization of virions from the cell surface. Once virus had entered the cell, it colocalized with markers of early endosomes (EEA1) and then late endosomes (LAMP-2). Inhibition of endosomal maturation-with siRNAs or dominant negative mutants targeting Rab5 and Rab7?inhibited infection and prevented release of viral RNA into the cell. These results indicate that EV7 is internalized by clathrin-mediated endocytosis and then moves to early and late endosomes before releasing its RNA. Trafficking through endosomes is known to be important for viruses that depend on low pH or endosomal cathepsin proteases to complete the entry process. However, we found that EV7 infection required neither low pH nor cathepsins.

IMPORTANCE The results demonstrate that echovirus 7 (EV7), after binding to decay-accelerating factor (DAF) on the cell surface, enters cells by clathrin-mediated endocytosis; this entry mechanism differs markedly from that of another DAF-binding enterovirus, coxsackievirus B3 (CVB3). Thus, after attachment to the same cell surface receptor, these closely related viruses enter the same cells by different mechanisms. The cellular cues required for release of viral RNA from the enterovirus capsid (“uncoating”) remain poorly defined. We found that EV7 moved to late endosomes and that release of RNA depended on endosomal maturation; nonetheless, EV7 did not depend on the endosomal factors implicated in uncoating and entry by other viruses. The results suggest either that an unidentified endosomal factor is essential for uncoating of EV7 or that trafficking through the endosome is an essential step in a pathway that leads to another intracellular organelle where uncoating is completed.

논문정보 F1000선정
- 형식: Research article
- 게재일: 2012년 04월 (BRIC 등록일 2012-08-07)
- 연구진: 국외연구진
- 추천: Faculty of 1000 Biology
- 추천사유: Faculty of 1000 evaluations, dissents and comments for [Kim C, Bergelson JM. Echovirus 7 entry into polarized intestinal epithelial cells requires clathrin and Rab7. MBio. 2012 Apr 10; 3(2):e00304-11; doi: 10.1128/mBio.00304-11]. Faculty of 1000,. F1000.com/717949001
Dot/Icm 제 4 유형 커플링 단백질 복합체의 선택적 이펙터 단백질 인식[Nat. Commun.]
김현민
발표: 김현민 (KAIST)
일자: 2020년 9월 29일 (화) 오후 02시 (한국시간)
언어: 한국어
참석자 접수신청하기
Massive Expansion of Functional Human iPSC-derived Cardiomyocytes for Industrial and Clinical Applications[Cell Stem Cell]
이소아
발표: 이소아 (Stanford Cardiovascular...)
일자: 2020년 10월 8일 (목) 오후 02시 (한국시간)
언어: 영어
참석자 접수신청하기
실제 세포밖 소포체 (엑소좀) 프로테옴: 단백질 분해 효소를 이용한 프로테옴 분석 [Journal of Extracellular Vesicles]
최동식
발표: 최동식 (Research Institute of th...)
일자: 2020년 10월 13일 (화) 오후 02시 (한국시간)
언어: 한국어
참석자 접수신청하기
면역항암제 치료에서 HLA-correcteted TMB 분석을 통한 기존 TMB 한계 극복 [Ann. Oncol.]
심준호
발표: 심준호 (성균관대학교 의과대학, 삼성서울병원)
일자: 2020년 10월 15일 (목) 오후 02시 (한국시간)
언어: 한국어
참석자 접수신청하기

  댓글 0
등록
 
목록
일루미나
관련링크
김천생 님 전체논문보기 >
관련인물
외부링크
Google (by Chonsaeng Kim)
Pubmed (by Chonsaeng Kim)
프리미엄 Bio일정 Bio일정 프리미엄 안내
[Webinar] Benchtop type으로 정확하고 경제적인 GenapSys NGS Sequencing System을 소개합니다.
제15회 KSGCT 정기학술대회/온라인
제15회 KSGCT 정기학술대회/온라인
초록접수: ~2020.10.08
사전접수: ~2020.10.23
날짜: 2020.11.05~06
Online 개최
대구경북과학기술원 (DGIST) 뇌·인지과학전공 온라인 전공설명회 개최
위로가기
한빛사 홈  |  한빛사FAQ  |  한빛사 문의 및 제안
 |  BRIC소개  |  이용안내  |  이용약관  |  개인정보처리방침  |  이메일무단수집거부
Copyright © BRIC. All rights reserved.  |  문의 member@ibric.org
트위터 트위터    페이스북 페이스북   유튜브 유튜브    RSS서비스 RSS
에펜도르프코리아 광고