한빛사 논문
Abstract
Young-Kook Kim1, Jinah Yeo1, Boseon Kim1, Minju Ha1 and V. Narry Kim1
1 School of Biological Sciences, Seoul National University, Seoul 151-742, Korea
Corresponding author : V. Narry Kim
Main Text
In our recent paper (Kim et al., 2011), we reported that a subset of microRNAs (miRNAs) including miR-141, miR-29b, miR-21, miR-106b, miR-15a, and miR-34a is decreased when cells are grown at low density or when cells are detached from a culture dish. Mutations at the center of miR-141 abolish such reduction. Based on these results, we proposed that some miRNAs are selectively destabilized depending on cell adhesion status and that the sequence element at the center of miR-141 serves as a decay signal. During follow-up study, however, we discovered that our results were due to specific loss of the miRNAs during RNA preparation using TRIzol, rather than intracellular miRNA regulation. Our current analyses indicate that structured small RNAs with low GC content are recovered inefficiently when a small number of cells is used for RNA isolation with TRIzol. We further find that, in addition to miRNAs, some pre-miRNAs, small interfering RNA (siRNA) duplexes, and transfer RNAs (tRNAs) are also extracted inefficiently under these conditions, reducing their representation in the pool of recovered RNAs. ....
논문정보
관련 링크
연구자 키워드
연구자 ID
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기