Jun-Seob Kim1, Paul Heo1, Tae-Jun Yang1, Ki-Sing Lee1, Da-Hyeong Cho1, Bum Tae Kim2, Ji-Hee Suh2, Hee-Jong Lim2, Dongwoo Shin3, Sung-Koo Kim4 and Dae-Hyuk Kweon1,*
1School of Life Science and Biotechnology and Center for Human Interface Nano Technology, Sungkyunkwan University, Suwon, Gyeonggi-do 440-746, South Korea
2Korea Research Institute of Chemical Technology, Daejeon 305-600, South Korea
3School of Medicine, Sungkyunkwan University, Suwon, Gyeonggi-do 440-746, South Korea
4Department of Biotechnology and Bioengineering, Pukyong National University, Busan 608-737, South Korea
We show that 3-[4-(4-methoxyphenyl)piperazin-1-yl]piperidin-4-yl biphenyl-4-carboxylate (C10), screened out of a chemical library, selectively kills bacterial persisters that tolerate antibiotic treatment but does not affect normal antibiotic-sensitive cells. C10 led persisters to antibiotic-induced cell death by causing reversion of persisters to antibiotic-sensitive cells. This work is the first demonstration in which the eradication of bacterial persisters is based on single-chemical supplementation. The chemical should be versatile in elucidating the mechanism of persistence.
* Corresponding author. Mailing address: School of Life Science and Biotechnology, Sungkyunkwan University, Suwon, Gyeonggi-do 440-746, South Korea.
† Supplemental material for this article may be found at http://aac.asm.org/.