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piggyBac transposition into primordial germ cells is an efficient tool for transgenesis in chickens

Tae Sub Park and Jae Yong Han1

World Class University Biomodulation Major, Department of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea

Transgenic birds embody one of the most potent and exciting research tools in biotechnology for agriculture, medicine, and model animals. To date, retrovirus- or lentivirus-mediated transgenesis has been established in chickens and quail. However, despite having a valid technique for viral transduction to achieve transgenic birds, many obstacles exist for practical applications because of relatively low and variable rates of germ-line transmission and transgenic offspring showing transgene silencing, as well as safety issues related to viral vector use. Thus, the generation of transgenic poultry by nonviral integration is a prerequisite for the introduction of biotechnology to practical applications. Herein, we show that a germ-line-competent chicken primordial germ-cell (PGC) line was established with high efficiency of transmission to offspring and that piggyBac transposition into PGCs improved the efficiency of transgenic chicken production and led to high-level transgene expression. GFP transgene-expressing donor PGC-transferred recipient chickens produced donor-derived progenies, and the germ-line transmission efficiency of donor PGCs was 95.2% on average. Subsequently, half of the donor-derived offspring (52.2%) were transgenic chicks because GFP-expressing donor PGCs, in which a transgene was inserted into one chromosome 20, were heterozygous. In all of the transgenic chickens, GFP expression was constant and strong, regardless of age. Our results demonstrate that piggyBac transposition into the chicken PGC line could be the surest way to generate transgenic chickens safely for practical applications.

germ-line chimera, piggyBac transposon, avian

1To whom correspondence should be addressed.

Author contributions: T.S.P. and J.Y.H. designed research; T.S.P. performed research; T.S.P. and J.Y.H. analyzed data; and T.S.P. and J.Y.H. wrote the paper.

- 형식: Research article
- 게재일: 2012년 05월 (BRIC 등록일 2012-05-30)
- 연구진: 국내연구진태극기
- 분야: Agriculture
관련 보도자료
광유전학의 과거, 현재와 미래[Neuron]
발표: 김윤석 (Stanford University)
일자: 2020년 7월 30일 (목) 오후 02시 (한국시간)
언어: 한국어
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  댓글 1
회원작성글 Todd Jun  (2012-06-06 03:49)
항상 열심히 하더니 훌륭한 저널에 논문 냈구나..정말 축하한다. 건강하구~~~
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