[DEBUG-WINDOW 처리영역 보기]
즐겨찾기  |  뉴스레터  |  오늘의 정보  |  e브릭몰e브릭몰 회원가입   로그인
BRIC홈 한국을 빛내는 사람들
2019 Bio Top5 인터뷰
스폰서배너광고 안내  배너1
전체보기 추천논문 상위피인용논문 인터뷰 그이후 한빛사통계
정승령 (Seung-Ryoung Jung) 저자 이메일 보기
조회 1746  인쇄하기 주소복사 트위터 공유 페이스북 공유 
DNA cleavage and opening reactions of human topoisomerase IIα are regulated via Mg2+-mediated dynamic bending of gate-DNA

Sanghwa Leea,b,1, Seung-Ryoung Junga,b,1, Kang Heoa,b, Jo Ann W. Bylc, Joseph E. Deweesec,d, Neil Osheroffc,e,2, and Sungchul Hohnga,f,b,2

aDepartment of Physics and Astronomy,
fBiophysics and Chemical Biology, and
bNational Center for Creative Research Initiatives, Seoul National University, Seoul 151-747, Korea;
cDepartments of Biochemistry and
eMedicine (Hematology/Oncology), Vanderbilt University School of Medicine, Nashville, TN 37232-0146; and
dDepartment of Pharmaceutical Sciences, Lipscomb University College of Pharmacy, Nashville, TN 37204-3951

Edited by Martin Gellert, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, and approved December 30, 2011 (received for review September 27, 2011)

Topoisomerase II resolves intrinsic topological problems of double-stranded DNA. As part of its essential cellular functions, the enzyme generates DNA breaks, but the regulation of this potentially dangerous process is not well understood. Here we report single-molecule fluorescence experiments that reveal a previously uncharacterized sequence of events during DNA cleavage by topoisomerase II: nonspecific DNA binding, sequence-specific DNA bending, and stochastic cleavage of DNA. We have identified unexpected structural roles of Mg2+ ions coordinated in the TOPRIM (topoisomerase-primase) domain in inducing cleavage-competent DNA bending. A break at one scissile bond dramatically stabilized DNA bending, explaining how two scission events in opposing strands can be coordinated to achieve a high probability of double-stranded cleavage. Clamping of the protein N-gate greatly enhanced the rate and degree of DNA bending, resulting in a significant stimulation of the DNA cleavage and opening reactions. Our data strongly suggest that the accurate cleavage of DNA by topoisomerase II is regulated through a tight coordination with DNA bending.

N-gate clamping, single-molecule FRET, type II topoisomerase, G-segment selection, indirect readout

1S.L. and S.-R.J. contributed equally to this work.

2To whom correspondence may be addressed.

Author contributions: S.L., S.-R.J., N.O., and S.H. designed research; S.L., S.-R.J., and K.H. performed research; J.A.W.B., J.E.D., N.O., and S.H. contributed new reagents/analytic tools; S.L., S.-R.J., and K.H. analyzed data; and S.L., S.-R.J., N.O., and S.H. wrote the paper.

- 형식: Research article
- 게재일: 2012년 02월 (BRIC 등록일 2012-02-10)
- 연구진: 국내(교신)+국외 연구진태극기
- 분야: Biophysics
경제적 의사결정의 개별과정을 동질한 성격의 뉴런으로 구현하기[Neuron]
발표: 유승범 (University of Minnesota)
일자: 2020년 1월 29일 (수) 오전 10시 (한국시간)
언어: 영어
참석자 접수신청하기
유전자 편집 기술을 활용한 도시농업 적합 작물 개발[Nat. Biotechnol.]
발표: 권춘탁 (Cold Spring Harbor Labor...)
일자: 2020년 2월 19일 (수) 오전 11시 (한국시간)
언어: 한국어
참석자 접수신청하기

  댓글 0
정승령 님 전체논문보기 >
김은지 (KAIST)
송지준 (KAIST)
이상화 (광주과학기술원 고등광기술연구...)
홍성철 (서울대학교)
관련분야 논문보기

Google (by Seung-Ryoung Jung)
Pubmed (by Seung-Ryoung Jung)
프리미엄 Bio일정 Bio일정 프리미엄 안내
[BRIC Webinar]경제적 의사결정의 개별과정을 동질한 성격의 뉴런으로 구현하기[Neuron]
[BRIC Webinar]Overview of NGS library Preparation[필코리아테크놀로지]
[BRIC Webinar]Overview of NGS library Preparation[필코리아테크놀로지]
사전접수: ~2020.01.21
날짜: 2020.01.22
Online 개최
[무료/ 취업연계]바이오QC 실무과정 8기 모집
[무료/ 취업연계]바이오QC 실무과정 8기 모집
사전접수: ~2020.02.10
날짜: 2020.03.02~06.12
장소: 성남시 분당구 황새울로
한빛사 홈  |  한빛사FAQ  |  한빛사 문의 및 제안
 |  BRIC소개  |  이용안내  |  이용약관  |  개인정보처리방침  |  이메일무단수집거부
Copyright © BRIC. All rights reserved.  |  문의 member@ibric.org
트위터 트위터    페이스북 페이스북   유튜브 유튜브    RSS서비스 RSS