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이정은
이정은 (Jungeun Lee) 저자 이메일 보기
서울대학교, Yale University
조회 7312  인쇄하기 주소복사 트위터 공유 페이스북 공유 
Analysis of Transcription Factor HY5 Genomic Binding Sites Revealed Its Hierarchical Role in Light Regulation of Development
Jungeun Lee 1, Kun He 2, Viktor Stolc 3, Horim Lee 4, Pablo Figueroa 5, Ying Gao 6, Waraporn Tongprasit 7, Hongyu Zhao 8, Ilha Lee 4*, and Xing Wang Deng 6

1 Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520-8104; Department of Biological Sciences, Seoul National University, Seoul 151-742, Korea
2 Peking-Yale Joint Center of Plant Molecular Genetics and Agrobiotechnology, Center for Bioinformatics, National Laboratory of Protein Engineering and Plant Genetic Engineering and College of Life Sciences, Peking University, Beijing 100871, People''s Republic of China
3 Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520-8104; Genome Research Facility, NASA Ames Research Center, Moffett Field, California 94035
4 Department of Biological Sciences, Seoul National University, Seoul 151-742, Korea
5 Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520-8104
6 Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520-8104; Peking-Yale Joint Center of Plant Molecular Genetics and Agrobiotechnology, Center for Bioinformatics, National Laboratory of Protein Engineering and Plant Genetic Engineering and College of Life Sciences, Peking University, Beijing 100871, People''s Republic of China
7 Eloret Corporation, Sunnyvale, California 94087
8 Department of Epidemiology and Public Health, School of Medicine, Yale University, New Haven, Connecticut 06520.

* To whom correspondence should be addressed.

The transcription factor LONG HYPOCOTYL5 (HY5) acts downstream of multiple families of the photoreceptors and promotes photomorphogenesis. Although it is well accepted that HY5 acts to regulate target gene expression, in vivo binding of HY5 to any of its target gene promoters has yet to be demonstrated. Here, we used a chromatin immunoprecipitation procedure to verify suspected in vivo HY5 binding sites. We demonstrated that in vivo association of HY5 with promoter targets is not altered under distinct light qualities or during light-to-dark transition. Coupled with DNA chip hybridization using a high-density 60-nucleotide oligomer microarray that contains one probe for every 500 nucleotides over the entire Arabidopsis thaliana genome, we mapped genome-wide in vivo HY5 binding sites. This analysis showed that HY5 binds preferentially to promoter regions in vivo and revealed >3000 chromosomal sites as putative HY5 binding targets. HY5 binding targets tend to be enriched in the early light-responsive genes and transcription factor genes. Our data thus support a model in which HY5 is a high hierarchical regulator of the transcriptional cascades for photomorphogenesis.

논문정보
- 형식: Research article
- 게재일: 2007년 03월 (BRIC 등록일 2009-11-17)
- 연구진: 국내(교신)+국외 연구진태극기
- 분야: Plant Science
- 피인용횟수: 최근 3년간 60회 이상 인용된 논문
RNA 기반의 합성생물학 유전자회로(synthetic gene circuits) 디자인[Nat. Chem. Biol.]
김종민
발표: 김종민 (POSTECH)
일자: 2020년 1월 6일 (월) 오후 02시 (한국시간)
언어: 영어
참석자 접수신청하기
수학울렁증에 대한 인지신경과학적 접근[Sci. Adv.]
최경환
발표: 최경환 (University of Chicago)
일자: 2019년 12월 19일 (목) 오후 02시 (한국시간)
언어: 한국어
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