Dr. Xinglu Huang1, Magdalena Swierczewska1,2, Dr. Ki Young Choi1, Dr. Lei Zhu1, Dr. Ashwinkumar Bhirde1, Dr. Jinwoo Park3, Dr. Kwangmeyung Kim4, Prof. Jin Xie5, Dr. Gang Niu1, Prof. Kang Choon Lee6, Dr. Seulki Lee1,*, Dr. Xiaoyuan Chen1,*
1Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
2Department of Biomedical Engineering, Stony Brook University (USA)
3BioActs, DKC Corporation (Korea)
4Center for Theragnosis, Korea Institute of Science and Technology (KIST, Korea)
5Department of Chemistry and Bio-Imaging Research Center, University of Georgia (USA)
6College of Pharmacy, SungKyunKwan University (Korea)
*Correspondence: Dr. Seulki Lee, Dr. Xiaoyuan Chen, Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
†This work was supported by the Intramural Research Program of the NIBIB, NIH and partially supported by an NIH Pathway to Independence (K99/R00) Award, the Henry M. Jackson Foundation, and the Intramural Research Program of KIST (Korea). We thank Myung Sun Lee for illustrations.
Turn off the lights! A universal nanoquencher that quenches a broad range of visible to near-infrared dyes by using a series of dark quenchers incorporated into a cell-permeable mesoporous silica nanoparticle has been developed. In combination with dye-labeled substrates, this nanoquencher boosts multiple fluorescence signals upon specific proteolysis, which allows real-time imaging of proteolytic cascades (see scheme).