Hyojin Kim1, Eunji Um2, Sung-Rae Cho3, Chorong Jung4, Hyongbum Kim4 & Jin-Soo Kim1
1Department of Chemistry, Seoul National University, Gwanak-gu, Seoul, South Korea. 2Cha Stem Cell Institute, Cha University, Seoul, South Korea. 3Department and Research Institute of Rehabilitation Medicine, Yonsei University College of Medicine, Seoul, South Korea. 4Graduate School of Biomedical Science and Engineering/College of Medicine, Hanyang University, Sungdong-gu, Seoul, South Korea. Correspondence should be addressed to J.-S.K., or Hyongbum K.
Zinc-finger nucleases (ZFNs) and TAL-effector nucleases (TALENs) are powerful tools for creating genetic modifications in eukaryotic cells and organisms. But wild-type and mutant cells that contain genetic modifications induced by these programmable nucleases are often phenotypically indistinguishable, hampering isolation of mutant cells. Here we show that transiently transfected episomal reporters encoding fluorescent proteins can be used as surrogate genes for the efficient enrichment of endogenous gene-modified cells by flow cytometry.