한빛사 논문
Abstract
Joonki Kim1,†, Jae-Hoon Jung1,†, Jose L. Reyes2, Youn-Sung Kim1, Sun-Young Kim1, Kyung-Sook Chung1, Jin A. Kim1, Minsun Lee1, Yoontae Lee3, V. Narry Kim3, Nam-Hai Chua2, Chung-Mo Park1,*
1Graduate School of Chemistry and Molecular Engineering, Seoul National University, Seoul 151-742, South Korea,
2Laboratory of Plant Molecular Biology, Rockefeller University, New York, New York 10021-3699, USA, and
3School of Biological Sciences, Seoul National University, Seoul, 151-742, South Korea
*Correspondence: Chung-Mo Park
Summary
Class III homeodomain-leucine zipper proteins regulate critical aspects of plant development, including lateral organ polarity, apical and lateral meristem formation, and vascular development. ATHB15, a member of this transcription factor family, is exclusively expressed in vascular tissues. Recently, a microRNA (miRNA) binding sequence has been identified in ATHB15 mRNA, suggesting that a molecular mechanism governed by miRNA binding may direct vascular development through ATHB15. Here, we show that miR166-mediated ATHB15 mRNA cleavage is a principal mechanism for the regulation of vascular development. In a gain-of-function MIR166a mutant, the decreased transcript level of ATHB15 was accompanied by an altered vascular system with expanded xylem tissue and interfascicular region, indicative of accelerated vascular cell differentiation from cambial/procambial cells. A similar phenotype was observed in Arabidopsis plants with reduced ATHB15 expression but reversed in transgenic plants overexpressing an miR166-resistant ATHB15. ATHB15 mRNA cleavage occurred in standard wheat germ extracts and in Arabidopsis and was mediated by miR166 in Nicotiana benthamiana cells. miR166-assisted ATHB15 repression is likely to be a conserved mechanism that regulates vascular development in all vascular plants.
Keywords:Arabidopsis;ATHB15;HD-ZIP;microRNA;mRNA cleavage;vascular development
논문정보
관련 링크
연구자 키워드
연구자 ID
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기