Seung Yun Yang,†,§ Sejin Son,‡,§ Sangsin Jang,† Hyunwoo Kim,‡ Gumhye Jeon,† Won Jong Kim,*,‡ and Jin Kon Kim*,†
†National Creative Research Center for Block Copolymer Self-Assembly, Department of Chemical Engineering, ‡Department of Chemistry, Pohang University of Science and Technology, Kyungbuk 790-784, Korea
We have developed ultrahigh density array of functionalized nanochannels by using a block copolymer having end di-COOH group. This approach provides a facile route for direct functionalization of wall surface of the nanochannels and immobilization site for molecular recognition agents (MRAs). By using overhanging single-stranded DNA as MRAs, the DNA-functionalized nanochannels showed high resolution to detect a single-base mismatch as well as to discriminate single-mismatched sequence at various locations by hybridization preference with MRAs.
Keywords: Single nucleotide polymorphisms (SNPs); functional nanochannels; molecular recognition agent; oligonucleotide; block copolymer