Seokjoong Kim1,3, Mi Jung Lee2,3, Hyojin Kim1, Mijin Kang2 & Jin-Soo Kim1
1Department of Chemistry, Seoul National University, Seoul, South Korea. 2ToolGen, Inc., Biotechnology Incubating Center, Seoul National University, Seoul, South Korea. 3These authors contributed equally to this work.
Correspondence to: Jin-Soo Kim
To the Editor:
Since the publication of our Correspondence1 and the reply of Joung et al.2, we improved zinc-finger nuclease (ZFN) modular assembly. ZFNs are artificial restriction enzymes3 composed of tailor-made zinc-finger DNA-binding arrays and the FokI nuclease domain, which can induce site-specific mutations4 and large chromosomal deletions5 in higher eukaryotic cells and organisms.