[DEBUG-WINDOW 처리영역 보기]
즐겨찾기  |  뉴스레터  |  오늘의 정보  |  e브릭몰e브릭몰 회원가입   로그인
BRIC홈 한국을 빛내는 사람들
물리적 거리두기 실천
스폰서배너광고 안내  배너1 배너2 배너3 배너4
LABox-과학으로 본 코로나19 (COVID-19)
전체보기 추천논문 상위피인용논문 인터뷰 그이후 한빛사통계
김문영 (Moon Young Kim) 저자 이메일 보기
조회 5424  인쇄하기 주소복사 트위터 공유 페이스북 공유 
Whole-genome sequencing and intensive analysis of the undomesticated soybean (Glycine soja Sieb. and Zucc.) genome

Moon Young Kima,1, Sunghoon Leeb,1,2, Kyujung Vana,1, Tae-Hyung Kimb,1,2, Soon-Chun Jeongc, Ik-Young Choid, Dae-Soo Kimb, Yong-Seok Leeb, Daeui Parkb, Jianxin Mae, Woo-Yeon Kimb, Byoung-Chul Kimb, Sungjin Parkb, Kyung-A Leeb, Dong Hyun Kima, Kil Hyun Kima, Jin Hee Shina, Young Eun Janga, Kyung Do Kima, Wei Xian Liua, Tanapon Chaisana, Yang Jae Kanga, Yeong-Ho Leea, Kook-Hyung Kimf, Jung-Kyung Moong, Jeremy Schmutzh, Scott A. Jacksone, Jong Bhakb,2,3, and Suk-Ha Leea,i,3

aDepartment of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea;
bKorean Bioinformation Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea;
cBio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk 363-883, Korea;
dNational Instrumentation Center for Environmental Management, Seoul National University, Seoul 151-921, Korea;
eDepartment of Agronomy, Purdue University, West Lafayette, IN 47906;
fDepartment of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea;
gRural Development Administration, Gyeonggi 441-770, Korea;
hHudsonAlpha Genome Sequencing Center, Huntsville, AL 35806; and
iPlant Genomics and Breeding Institute, Seoul National University, Seoul 151-921, Korea

Edited* by Ronald L. Phillips, University of Minnesota, St. Paul, MN, and approved October 29, 2010 (received for review July 12, 2010)

The genome of soybean (Glycine max), a commercially important crop, has recently been sequenced and is one of six crop species to have been sequenced. Here we report the genome sequence of G. soja, the undomesticated ancestor of G. max (in particular, G. soja var. IT182932). The 48.8-Gb Illumina Genome Analyzer (Illumina-GA) short DNA reads were aligned to the G. max reference genome and a consensus was determined for G. soja. This consensus sequence spanned 915.4 Mb, representing a coverage of 97.65% of the G. max published genome sequence and an average mapping depth of 43-fold. The nucleotide sequence of the G. soja genome, which contains 2.5 Mb of substituted bases and 406 kb of small insertions/deletions relative to G. max, is ∼0.31% different from that of G. max. In addition to the mapped 915.4-Mb consensus sequence, 32.4 Mb of large deletions and 8.3 Mb of novel sequence contigs in the G. soja genome were also detected. Nucleotide variants of G. soja versus G. max confirmed by Roche Genome Sequencer FLX sequencing showed a 99.99% concordance in single-nucleotide polymorphism and a 98.82% agreement in insertion/deletion calls on Illumina-GA reads. Data presented in this study suggest that the G. soja/G. max complex may be at least 0.27 million y old, appearing before the relatively recent event of domestication (6,000∼9,000 y ago). This suggests that soybean domestication is complicated and that more in-depth study of population genetics is needed. In any case, genome comparison of domesticated and undomesticated forms of soybean can facilitate its improvement.
massively parallel sequencing, sequence variation, wild soybean, divergence, genome duplication

3To whom correspondence may be addressed.

Author contributions: M.Y.K., K.V., S.-C.J., J.M., J.B., and S.-H.L. designed research; I.-Y.C., D.-S.K., D.H.K., K.H.K., J.H.S., Y.E.J., K.D.K., W.X.L., T.C., and Y.-H.L. performed research; S.L., T.-H.K., D.-S.K., Y.-S.L., D.P., W.-Y.K., B.-C.K., S.P., K.-A.L., and Y.J.K. analyzed data; and M.Y.K., S.L., K.V., T.-H.K., S.-C.J., K.-H.K., J.-K.M., J.S., S.A.J., J.B., and S.-H.L. wrote the paper.

1M.Y.K., S.L., K.V., and T.-H.K. contributed equally to this work.

2Present address: Personal Genomics Institute, Suwon, Gyeonggi 433-759, Korea.

*This Direct Submission article had a prearranged editor.

The authors declare no conflict of interest.

Database deposition: The sequence data from this study have been deposited in the National Center for Biotechnology Information Short Read Archive, www.ncbi.nlm.nih.gov/Traces/sra/sra.cgi (accession no. SRA009252).

This article contains supporting information online at

- 형식: Research article
- 게재일: 2010년 12월 (BRIC 등록일 2010-12-06)
- 연구진: 국내(교신)+국외 연구진태극기
- 분야: Agriculture
관련 보도자료
세포 형광 이미징 멀티플렉싱을 위한 초고속 사이클링[Angew. Chem.-Int. Edit.]
발표: 고진아 (Massachusetts General Ho...)
일자: 2020년 4월 14일 (화) 오전 10시 (한국시간)
언어: 영어
참석자 접수신청하기
The presenting symptoms of cancer and stage at diagnosis[Lancet Oncol.]
발표: 구민정 (University College Londo...)
일자: 2020년 4월 20일 (월) 오후 04시 (한국시간)
언어: 영어
참석자 접수신청하기

  댓글 0
김문영 님 전체논문보기 >
김태형 (국가생물자원정보관리센터, 현...)
박종화 (클리노믹스(주))
반규정 (서울대학교)
이석하 (서울대학교)
이성훈 ((주)테라젠이텍스)
관련분야 논문보기

Google (by Moon Young Kim)
Pubmed (by Moon Young Kim)
프리미엄 Bio일정 Bio일정 프리미엄 안내
[BRIC Webinar]세포 형광 이미징 멀티플렉싱을 위한 초고속 사이클링[Angew. Chem.-Int. Edit.]
[재직자무료교육] 분석기기(HPLC) 활용 품질검사 실습(4.9~4.10) / 화장품 제형제조 기술 실습(4.13~4.14)
[재직자무료교육] 분석기기(HPLC) 활용 품질검사 실습(4.9~4.10) / 화장품 제형제조 기술 실습(4.13~4.14)
날짜: 2020.04.09~14
장소: 충청북도 청주시 흥덕구 오송읍 오송생명1로 194-41 충북산학융합본부 기업연구1관 교육장
[BRIC Webinar]새로운 염기서열 분석방법(BTSeq)을 이용한 hCoV-19 전장유전체 염기서열의 동정 [CELEMICS]
한빛사 홈  |  한빛사FAQ  |  한빛사 문의 및 제안
 |  BRIC소개  |  이용안내  |  이용약관  |  개인정보처리방침  |  이메일무단수집거부
Copyright © BRIC. All rights reserved.  |  문의 member@ibric.org
트위터 트위터    페이스북 페이스북   유튜브 유튜브    RSS서비스 RSS