Jinwoo Lee 1,‡, Sanghwa Lee 1,‡, Kaushik Ragunathan2, Dr. Chirlmin Joo3, Prof. Dr. Taekjip Ha4, Prof. Dr. Sungchul Hohng1
1Department of Physics and Astronomy, Department of Biophysics and Chemical Biology, National Center for Creative Research Initiatives, Seoul National University, Seoul 151-747 (Korea), Fax: (+82) 2-871-8083
2Center for Biophysics and Computational Biology, Department of Physics, Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, IL 61801 (USA)
3School of Biological Sciences, Seoul National University, Seoul 151-747 (Korea)
4Center for Biophysics and Computational Biology, Department of Physics, Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Howard Hughes Medical Institute, Urbana, IL 61801 (USA)
Correspondence: Prof. Dr. Sungchul Hohng
†This work was supported by the Creative Research Initiatives (Physical Genetics Laboratory, 2009-0081562), the World Class University project (R31-2009-100320), and the US National Science Foundation grants (0822613 and 0646550). T.H. is an investigator with the Howard Hughes Medical Institute. J.L. was financially supported by a Hi Seoul Science (Humanities) Fellowship from Seoul Scholarship Foundation.
‡These authors contributed equally to this work.
Six interfluorophore FRET efficienciesEij (see scheme) are determined in real time by a single-molecule four-color FRET technique both in confocal and in total-internal-reflection fluorescence microscopy. This technique was used to probe the correlated motion of the four arms of the Holliday junction, and to assess correlation of RecA-mediated strand exchange events at both ends of a synaptic complex.
Keywords:conformational dynamics;DNA structures;fluorescence;FRET;single‒molecule studies