Sung Tae Kim†§, Dong-Joo Kim‡§, Tae-Jin Kim†, Deok-Won Seo‡, Tae-Hong Kim‡, Seung-Yong Lee‡, Kwanghee Kim†, Kyung-Mi Lee*† and Sang-Kwon Lee*‡
† Global Research Laboratory, Department of Biochemistry, Division of Brain Korea, College of Medicine, Korea University, Seoul 136-701, Korea
‡ Department of Semiconductor Science and Technology, Chonbuk National University, Jeonju 561-756, Korea
* To whom correspondence should be addressed. (S.-K.L.) Tel: +82-63-270-3973. Fax: +82-63-270-3585. (K.-M.L.) Tel: +82-2-920-6251. Fax: +82-2-920-6252., § These authors contributed equally to this study.
Silicon nanowires (SiNWs) offer promising inorganic nanostructures for biomedical application. Here, we report the development of a novel SiNW array designed for isolating primary CD4+ T lymphocytes from the heterogeneous mixture of cell populations. Our system employed the specific high-affinity binding features of streptavidin (STR)-functionalized SiNW with biotin-labeled CD4+ T lymphocytes. Fabricated SiNW arrays easily separated the CD4+ T lymphocytes from the mouse whole splenocytes with over ~88% purity and demonstrated tight attachment to CD4+ T lymphocytes by scanning electron microscopy. Thus, our STR-SiNW arrays provide a potential tool for specific cell separation and further present a possibility to be applied to the other area of biomedical applications.
Keywords:Silicon nanowire; streptavidin; CD4+ T lymphocyte; cell separation