한빛사 논문
Abstract
Jin-Won Youn 1, 5, Su-Hyung Park 1, Dimitri Lavillette 2, Francois-Loic Cosset 2, Se-Hwan Yang 1, Chang Geun Lee 1, Hyun-Tak Jin 1, Chang-Min Kim 3, Mohamed Tarek M. Shata 4, Dong-Hun Lee 5, Wolfram Pfahler 5, Alfred M. Prince 5, Young Chul Sung 1 *§
1National Research Laboratory of DNA Medicine, Division of Molecular and Life Science, POSTECH Biotech Center, Pohang University of Science and Technology, Pohang, Republic of Korea
2Laboratoire de Vectorologie Retrovirale et Therapie Genique, INSERM U412/IFR128 BioSciences Lyon-Gerland, ENS de Lyon, Lyon, France
3Research Institute, National Cancer Center, Goyang, Republic of Korea
4Viral Immunology Laboratory, Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, OH
5The Laboratory of Virology, the Lindsley F. Kimball Research Institute of the New York Blood Center, New York, NY
*Correspondence to Young Chul Sung, Division of Molecular and Life Sciences, POSTECH Biotech Center, Pohang University of Science and Technology, San 31, Hyojadong, Namgu, Pohang, 790-784, Republic of Korea
J.-W.Y. and S.-H.P. contributed equally to this work.
§fax: (82) 54-279-5544
Abstract
Immune correlates of protection against hepatitis C virus (HCV) infection are not well understood. Here we investigated 2 naive and 6 immunized chimpanzees before and after intravenous challenge, 12 weeks after the last immunization, with 100 50% chimpanzee infectious doses (CID(50)) of heterologous genotype 1b HCV. Vaccination with recombinant DNA and adenovirus vaccines expressing HCV core, E1E2, and NS3-5 genes induced long-term HCV-specific antibody and T-cell responses and reduced peak viral load about 100 times compared with controls (5.91 +/- 0.38 vs. 3.81 +/- 0.71 logs, respectively). There was a statistically significant inverse correlation between peak viral loads and envelope glycoprotein 2 (E2)-specific antibody responses at the time of challenge. Interestingly, one vaccinee that had sterilizing immunity against slightly heterologous virus generated the highest level of E2-specific total and neutralizing antibody responses as well as strong NS3/NS5-specific T-cell proliferative responses. The other four vaccinees with low levels of E2-specific antibody had about 44-fold reduced peak viral loads but eventually developed persistent infections. In conclusion, vaccine-induced E2-specific antibody plays an important role in prevention from nonhomologous virus infection and may provide new insight into the development of an effective HCV vaccine. (HEPATOLOGY 2005;42:1429-1436.).
논문정보
관련 링크
연구자 키워드
연구자 ID
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기