Mi-Sook Lee,¹ Tae Young Kim,¹ Yong-Bae Kim,² Sung-Yul Lee,¹ Seong-Gyu Ko,^2,+ Hyun-Soon Jong,² Tae-You Kim,^1,2 Yung-Jue Bang,^1,2 and Jung Weon Lee^1,2*
Cancer Research Institute, Departments of Molecular and Clinical Oncology and,¹ Tumor Biology, College of Medicine, Seoul National University, Seoul 110-799, Republic of Korea²

Integrin-mediated cell adhesion and spreading enables cells to respond to extracellular stimuli for cellular functions. Using a gastric carcinoma cell line that is usually round in adhesion, we explored the mechanisms underlying the cell spreading process, separate from adhesion, and the biological consequences of the process. The cells exhibited spreading behavior through the collaboration of integrin-extracellular matrix interaction with a Smad-mediated transforming growth factor ß1 (TGFß1) pathway that is mediated by protein kinase C (PKC). TGFß1 treatment of the cells replated on extracellular matrix caused the expression and phosphorylation of PKC, which is required for expression and activation of integrins. Increased expression of integrins 2 and 3 correlated with the spreading, functioning in activation of focal adhesion molecules. Smad3, but not Smad2, overexpression enhanced the TGFß1 effects. Furthermore, TGFß1 treatment and PKC activity were required for increased motility on fibronectin and invasion through matrigel, indicating their correlation with the spreading behavior. Altogether, this study clearly evidenced that the signaling network, involving the Smad-dependent TGFß pathway, PKC expression and phosphorylation, and integrin expression and activation, regulates cell spreading, motility, and invasion of the SNU16mAd gastric carcinoma cell variant.

^* Corresponding author. Mailing address: Cancer Research Institute, College of Medicine, Seoul National University, 28, Yongon-dong, Chongno-gu, Seoul 110-799, Republic of Korea. Phone: 82-2-3668-7030. Fax: 82-2-766-4487. E-mail: jwl@snu.ac.kr.

⁺ Present address: Laboratory of Clinical Biology and Pharmacogenomics, Department of Preventive Medicine, School of Oriental Medicine, Kyunghee University, Seoul 130-701, Republic of Korea.

[밀테니바이오텍] 새로운 대리점 소식!! - 서울/경기 총판 대리점 "SureBio" 02-6274-0700

[클리노믹스] QUICK & EASY NGS Service - High Quality, Quick Result Service, Priced Low!!

[ASSIST-BIO] 바이오전문 PPT 템플릿, 논문그림 탬플릿 주문제작 - 이곳에서 다운로드 하세요!

[SARTORIUS] 150년 전통의 바이오 공정 전문 기업 - 바이오 의약품 연구개발 및 통합된 기반 기술 제공!!

[에이프런티어]single-cell수준까지 캡처 가능한 Laser Capture Microdissection 장비 - 20%할인 프로모션

[에펜도르프코리아] Your Lifelong Companion - Eppendorf 최신 마이크로 냉장원심분리기 5425R 출시!

[케미스카이] 자동멀티반응시스템, 1개의 시스템으로 4개의 반응을 동시에!!