Park F. Cho,
1 Francis Poulin,
1,
4,* Yoon Andrew Cho-Park,
1,
3 Ian B. Cho-Park,
1,
3 Jarred D. Chicoine,
2 Paul Lasko,
2 and Nahum Sonenberg
1,*
1 Department of Biochemistry, McGill Cancer Center, McGill University, 3655 Promenade Sir William Osler, Montréal, Québec H3G 1Y6, Canada
2 Department of Biology, McGill University, 1205 Avenue Dr. Penfield, Montréal, Québec H3A 1B1, Canada
Correspondence:
Francis Poulin
Phone: (510) 643-6227; Fax: (510) 643-5022 [F.P.]
Correspondence:
Nahum Sonenberg
Phone: (514) 398-7274; Fax: (514) 398-1287 [N.S.]
Translational control is a key genetic regulatory mechanism implicated in regulation of cell and organismal growth and early embryonic development. Initiation at the mRNA 5'' cap structure recognition step is frequently targeted by translational control mechanisms. In the Drosophila embryo, cap-dependent translation of the uniformly distributed caudal (cad) mRNA is inhibited in the anterior by Bicoid (Bcd) to create an asymmetric distribution of Cad protein. Here, we show that d4EHP, an eIF4E-related cap binding protein, specifically interacts with Bcd to suppress cad translation. Translational inhibition depends on the Bcd binding region (BBR) present in the cad 3'' untranslated region. Thus, simultaneous interactions of d4EHP with the cap structure and of Bcd with BBR renders cad mRNA translationally inactive. This example of cap-dependent translational control that is not mediated by canonical eIF4E defines a new paradigm for translational inhibition involving tethering of the mRNA 5'' and 3'' ends.
Footnotes
3 These authors contributed equally to this work.
4 Present address: Department of Integrative Biology, University of California, Berkeley, Berkeley, California 94720.
e브릭몰 
Google (by Park F. Cho)
Pubmed (by Park F. Cho)
![[BRIC Webinar]수학울렁증에 대한 인지신경과학적 접근[Sci. Adv.]](/upload/board/files/bioschedule_pri/pri_0239652.gif)
![[무료/취업연계]바이오QC 실무과정 8기 모집](/upload/board/files/bioschedule_pri/pri_0239402.jpg)
