실험Q&A를 통해 여러분의 지식을 나누어 주세요. 답변을 등록하시려면 로그인 해주세요.
본 정보는 네티즌에 의해 작성된 정보로, 내용 중 중요하다고 생각되는 부분은 추가적인 사실 확인을 반드시 하시길 바랍니다.
transfection 한 DNA 가 암세포의 성장을 느리게하는 역할을 할 수 도 있지 않는건 아닌가요?
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레벨1
Q.E.D.
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22.05.26 18:52
시간이 없어서 짧게 답변 합니다.
혹시 transfection시에 lipofectamin을 사용하시나요?
아니면 전기 자극으로 주입하시나요?
lipofectamin의 경우 양적으로 독성이 있고
전기자극의 경우 시료에 거품이 많이 형성되면
세포 독성이 있습니다. 이런 부분도 확인해 보세요!
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레벨3
아스널
(대학원생)
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22.05.26 19:05
Q.E.D.님, 저는 lipofectamine으로 transfection합니다.
5 ug DNA 기준으로 10 uL 그리고 lipofectamine 7.5 uL 넣습니다. 참고로 lipofectamine 3000 ThermoFisher Scientific kit 사용 중입니다. 감사합니다.
lenti가 plasmid 이름인가요?
보통 lenti virus는 transduction인데요..
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레벨2
rlaeheod
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22.05.31 13:25
Cell confluency를 어떻게 하셨는지요?<br />
6well 기준으로 진행하신건가요? 100pi dish에 진행하신건가요?
transfection이 보통 세포가 천천히 자라게끔하지만, 아예 안자란다고 느껴지는경우라면 뭔가 문제가 생긴것같습니다.
lipofectamine을 사용하셨다고 하셨는데, 요즘은 독성이 심하지 않아서 거의 크게 영향을 안준다고 하더라구요.
lipofectamine과 DNA ratio가 중요한데 DNA : lipo 비율을 1: 2~3정도로 권장하는데 잘 맞춰서 진행하신 것 같구요.
GFP같은거로 transfection efficiency부터 체크하시는걸 추천드립니다.
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biosolyx19
(비회원)
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22.06.13 16:10
<p style="-webkit-text-stroke-width:0px; text-align:start; text-indent:0px"><span style="font-size:14px"><span style="color:#444444"><span gothic="" malgun=""><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial"><span style="font-size:14px"><span style="color:#444444"><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial"><span style="font-size:12px"><span style="color:#444444"><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial"><span style="font-size:14px"><span style="word-break:break-all"><span style="font-size:14px"><span style="color:#444444"><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial">안녕하세요.</span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>
<p style="-webkit-text-stroke-width:0px; text-align:start; text-indent:0px"><span style="font-size:14px"><span style="color:#444444"><span gothic="" malgun=""><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial"><span style="font-size:14px"><span style="color:#444444"><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial"><span style="font-size:12px"><span style="color:#444444"><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial"><span style="font-size:14px"><span style="word-break:break-all"><span style="font-size:14px"><span style="color:#444444"><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial">유전자 전달체 연구개발 기업 (주)</span></span></span></span></span></span></span></span></span></span></span></span></span></span><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial"><strong>바이오솔릭스</strong> 입</span></span></span></span></span></span></span></span></span></span></span></span></span><span style="color:#444444"><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial">니다.</span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>
<p style="-webkit-text-stroke-width:0px; text-align:start; text-indent:0px"><span style="font-size:14px"><span style="color:#444444"><span gothic="" malgun=""><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial">transfection을 한다고 해서 세포 생장 속도를 크게 더디게 만들지 않습니다.</span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>
<p style="-webkit-text-stroke-width:0px; text-align:start; text-indent:0px"><span style="font-size:14px"><span style="color:#444444"><span gothic="" malgun=""><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial">사용량이 많다면 오히려 세포 독성이 있겠지요.</span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>
<p style="-webkit-text-stroke-width:0px; text-align:start; text-indent:0px">
<p style="-webkit-text-stroke-width:0px; text-align:start; text-indent:0px"><span style="color:#444444"><span style="font-size:14px">올려주신 사진의 4번째가 negative control 인 것 같고</span></span>
<p style="-webkit-text-stroke-width:0px; text-align:start; text-indent:0px"><span style="color:#444444"><span style="font-size:14px">x4, x10 은 확대 배율인것이라고 생각하면</span></span>
<p style="-webkit-text-stroke-width:0px; text-align:start; text-indent:0px"><span style="color:#444444"><span style="font-size:14px">올려주신 x10 사진은 무언가 이상합니다.</span></span>
<p style="-webkit-text-stroke-width:0px; text-align:start; text-indent:0px"><span style="color:#444444"><span style="font-size:14px">세포가 아니고 무언가 다 떠잇는 세포 같습니다.</span></span>
<p style="-webkit-text-stroke-width:0px; text-align:start; text-indent:0px">transfection시 사용하는 핵산의 양과 전달체의 양을 다시 체크해보세요.
<p style="-webkit-text-stroke-width:0px; text-align:start; text-indent:0px">
<p style="-webkit-text-stroke-width:0px; text-align:start; text-indent:0px"><span style="font-size:14px"><span style="color:#444444"><span gothic="" malgun=""><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial">그리고 </span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span><span style="font-size:14px"><span style="color:#444444"><span gothic="" malgun=""><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial"><span style="font-size:14px"><span style="color:#444444"><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial"><span style="font-size:12px"><span style="color:#444444"><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial"><span style="font-size:14px"><span style="word-break:break-all"><span style="font-size:14px"><span style="color:#444444"><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial">당사는 pDNA, siRNA, mRNA 등 다양한 핵산 전달체를 전달하는 제품을 lipofectamine을 대체하여 국산화 하기 위해 연구 개발 중이며, <span style="color:red"><strong>Solfect</strong></span> 시리즈의 제품을 출시 하였습니다.</span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>
<p style="-webkit-text-stroke-width:0px; text-align:start; text-indent:0px"><span style="font-size:14px"><span style="color:#444444"><span gothic="" malgun=""><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial"><span style="font-size:14px"><span style="color:#444444"><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial"><span style="font-size:12px"><span style="color:#444444"><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial"><span style="color:#444444"><span style="font-size:14px">또한 새롭게 개발한 <span style="color:red"><strong><span style="color:RED"><strong>transfection</strong></span></strong></span> 시약 (solfect series)는 일반적으로 FBS가 있는 media에 처리 하여도 무방 합니다.</span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>
<p style="-webkit-text-stroke-width:0px; text-align:start; text-indent:0px"><span style="font-size:14px"><span style="color:#444444"><span gothic="" malgun=""><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial"><span style="font-size:14px"><span style="color:#444444"><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial"> <span style="font-size:12px"><span style="color:#444444"><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial"><span style="font-size:14px"><span style="word-break:break-all"><span style="font-size:14px"><span style="color:#444444"><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial">무료 샘플을 원하시거나, <span style="color:red"><strong><span style="color:red"><strong><span style="color:RED"><strong>Transfection</strong></span></strong></span></strong></span>에 관한 의문점이나 프로토콜, 기타 유전자 전달에 관련된 전반적인 실험에 고민이 있으시면 support@biosolyx.com 으로 연락 부탁 드립니다.</span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>
<p style="-webkit-text-stroke-width:0px; text-align:start; text-indent:0px"><span style="font-size:14px"><span style="color:#444444"><span gothic="" malgun=""><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial"><span style="font-size:14px"><span style="color:#444444"><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial"><span style="font-size:12px"><span style="color:#444444"><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial"><span style="font-size:14px"><span style="word-break:break-all"><span style="font-size:14px"><span style="color:#444444"><span style="font-style:normal"><span style="font-variant-ligatures:normal"><span style="font-variant-caps:normal"><span style="font-weight:400"><span style="letter-spacing:normal"><span style="orphans:2"><span style="text-transform:none"><span style="white-space:normal"><span style="widows:2"><span style="word-spacing:0px"><span style="text-decoration-thickness:initial"><span style="text-decoration-style:initial"><span style="text-decoration-color:initial">바이오솔릭스 드림</span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>