실험 Q&A > Buffer/Reagent
5-(and-6)-carboxy SNARF-1 (Thermo)를 사용해 염색하는데요 도와주세요
레벨2 뿌압뿌양빠앙 (대학원생)
한 바이엘당 50 µg ,
These conjugates can be dis solved in userspec i fied intracellular injection buffers at 10 mM (=100 mg/mL for a 10,000 MW dextran) for microinjection into cells.
Optimal loading conditions for each cell type and ex per i ment should be determined by the researcher. The literature cites a wide range of loading conditions, from 1 to 20 µM SNARF AM acetate incubat ed with cells for from 10 to 60 min utes. DMSO stock so lu tions are typically diluted at least 1:1000 into load ing buffer to reduce the exposure of cells to DMSO, and the loading buffer should be se rum-free because serum often con tains es terase ac tiv i ty.
이렇게 되어있는데 DMSO 1ml에 녹인 후 pbs로 희석, incom으로 농도 맞춰 셀에 주고있느데
염색이 너무 지저분하게 됩니다. 부분부분 진한 빨간점들이 보여서 데이터로 쓸수없어요ㅠㅠ 희석이 잘못된걸까요 10uM로 계산식 부탁드려요ㅠㅠ
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