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IL-6 kit assay
레벨2 가즈아분자생물 (대학원생)
IL-6 kit protocol 정리하고 있는데 영어가 많이 미흡합니다..
3. Standard dilution on the microwell plate (Alternatively the standard dilution can be prepared in tubes - see “External standard dilution“ on page 3): Add 100 μL of Sample Diluent in duplicate to all standard wells. Pipette 100 μL of prepared standard (see Preparation of Standard “Mouse IL-6 standard“ on page 3, concentration = 4000.0 pg/mL), in duplicate, into well A1 and A2 (see Table 1). Mix the contents of wells A1 and A2 by repeated aspiration and ejection (concentration of standard 1 S1 = 2000 pg/mL), and transfer 100 μL to wells B1 and B2, respectively (see Figure 7). Take care not to scratch the inner surface of the microwells. Continue this procedure 5 times, creating two rows of mouse IL-6 standard dilutions, ranging from 2000.0 to 31.3 pg/mL. Discard 100 μL of the contents from the last microwells (G1, G2) used. In case of an external standard dilution (see “External standard
dilution“ on page 3), pipette 100 μL of these standard dilutions
4. Add 100 μL of Sample Diluent in duplicate to the blank wells.
5. Add 50 μL of Sample Diluent to the sample wells.
6. Add 50 μL of each sample in duplicate to the sample wells.
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